|Application ||WB, IHC-P, E|
|Other Accession||NP_003147, 21070997|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 59, 75, 87 kDa |
Observed: 88 kDa
|Application Notes||STIM1 antibody can be used for detection of STIM1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL.|
|Other Names||STIM1 Antibody: GOK, TAM, IMD10, D11S4896E, GOK, Stromal interaction molecule 1, stromal interaction molecule 1|
|Target/Specificity||STIM1; At least two isoforms of STIM1 are known to exist; this antibody will detect only the larger form. This STIM1 antibody is predicted to have no cross-reactivity to STIM2.|
|Reconstitution & Storage||STIM1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||STIM1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Cytoplasm, cytoskeleton. Note=Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium and is detected at punctae corresponding to junctions between the endoplasmic reticulum and the cell membrane. Associated with the microtubule network at the growing distal tip of microtubules|
|Tissue Location||Ubiquitously expressed in various human primary cells and tumor cell lines.|
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Provided below are standard protocols that you may find useful for product applications.
STIM1 Antibody: In T lymphocytes, the sole pathway for Ca2+ entry following antigen-receptor binding is through store-operated Ca2+-release-activated Ca2+ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca2+ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca2+ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca2+ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca2+ entry.
Luik RM and Lewis RS. New insights into the molecular mechanisms of store-operated Ca2+ signaling in T cells. Trends Mol. Med. 2007; 13:103-7.
Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
Zhang SL, Yu Y, Roos J, et al. STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane. Nature 2005; 437:902-5.
Spassova MA, Soboloff J, He L-P, et al. STIM1 has a plasma membrane role in the activation of store-operated Ca2+ channels. Proc. Natl. Acad. Sci. USA 2006; 103:4040-5.
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