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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   KIF5 Antibody   

KIF5 Antibody

     
  • WB - KIF5 Antibody ASC10565
    Western blot analysis of C-raf in A431 cell lysate with NPC1 antibody at (A) 0.5 and (B) 1 µg/mL.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, E
Primary Accession Q12840
Other Accession Q12840, 3798
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 114 kDa

Observed: 116 kDa
Application Notes KIF5 antibody can be used for detection of KIF5 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL and Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Additional Information
Gene ID 3798
Target/Specificity KIF5 antibody was raised against a 20 amino acid synthetic peptide from near the carboxy terminus of human KIF5.

The immunogen is located within amino acids 880 - 930 of KIF5.
Reconstitution & Storage KIF5 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsKIF5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KIF5A
Synonyms NKHC1
Function Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. The ZFYVE27-KIF5A complex contributes to the vesicular transport of VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 proteins in neurons.
Cellular Location Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Note=Concentrated in the cell body of the neurons, particularly in the perinuclear region
Tissue Location Distributed throughout the CNS but is highly enriched in subsets of neurons
Research Areas
Citations (0)

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Background

KIF5 Antibody: The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.

References

Hirokawa N. mRNA transport in dendrites: RNA granules, motors and tracks. J. Neurosci. 2006; 26:7139-42.
Niclas J, Navone F, Hom-Booher N, et al. Cloning and localization of a conventional KIF5 motor expressed exclusively in neurons. Neuron 1994; 12:1059-72.
Fichera M, Lo Giudice M, Falco M, et al. Evidence of kinesin heavy chain (KIF5A) involvement in pure hereditary spastic paraplegia. Neurology 2004; 63:1108-10.

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$ 320.00
Cat# ASC10565
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