|Application ||WB, IHC-P, E|
|Other Accession||AAH71557, 47938093|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||118392 Da|
|Application Notes||PTK7 antibody can be used for detection of PTK7 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL.|
|Reconstitution & Storage||PTK7 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||PTK7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.|
|Cellular Location||Membrane; Single-pass type I membrane protein Cell junction. Note=Colocalizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells|
|Tissue Location||Highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon|
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Provided below are standard protocols that you may find useful for product applications.
PTK7 Antibody: Protein-tyrosine kinases (PTKs) play important roles in regulating cell proliferation and differentiation during development. One member of the PTK family, PTK7, has been suggested to regulate the planar cell polarity (PCP) pathway in vertebrates and may play a role in neural convergent extension and neural tube closure. PTK7 has also been implicated in the development of cancer. Loss of PTK7 expression was seen in several melanoma cell lines and biopsies. Conversely, high-throughput analysis of acute myeloid leukemia samples showed an increased level of PTK7 expression compared to normal bone marrow and purified CD34+ cells. Multiple isoforms of PTK7 are known to exist.
Park SK, Lee HS and Lee ST. Characterization of the human full-length PTK7 cDNA encoding a receptor protein kinase-like molecule closely related to chick KLG. J. Biochem.1996; 119:235-9.
Lu X, Borchers AG, Jolicoeur C, et al. PTK7/CCK-4 is a novel regulator of planar cell polarity in vertebrates. Nature2004; 430:93-8.
Easty DJ, Mitchell PJ, Patel K, et al. Loss of expression of receptor tyrosine kinase family genes PTK7 and SEK in metastatic melanoma. Int. J. Cancer1997; 1061-5.
Muller-Tidow C, Schwable J, Steffen B, et al. High-throughput analysis of genome-wide receptor tyrosine kinase expression in human cancers identifies potential novel drug targets. Clin. Cancer Res.2004; 10:1241-9.
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