|Application ||WB, IHC-P, IF, E|
|Other Accession||AAH95421, 127801437|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 55 kDa |
Observed: 51 kDa
|Application Notes||STEAP3 antibody can be used for detection of STEAP3 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||STEAP3; This STEAP3 antibody does not cross-react with other STEAP proteins.|
|Reconstitution & Storage||STEAP3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||STEAP3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NADP(+) as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.|
|Cellular Location||Endosome membrane; Multi-pass membrane protein. Note=Localizes to vesicular-like structures at the plasma membrane and around the nucleus|
|Tissue Location||Expressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma.|
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Provided below are standard protocols that you may find useful for product applications.
STEAP3 Antibody: The six-transmembrane epithelial antigen of prostate 3 (STEAP3) is a member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. Similar to two other members of the STEAP family (STEAP 2 and STEAP4), STEAP3 promotes both iron and copper reduction. STEAP3 is highly expressed in hematopoietic tissues and colocalizes with the transferrin endosome. Overexpression of STEAP3 stimulates iron reduction; mice lacking STEAP3 are deficient in erythroid ferrireductase activity, suggesting that STEAP3 is an endosomal ferrireductase required for transferrin-dependent iron uptake in erythroid cells.
Ohgami RS, Campagna DR, Greer EL, et al. Identification of a ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Nat. Genet.2005; 37:1264-9.
Ohgami RS, Campagna DR, McDonald A, et al. The Steap proteins are metalloreductases. Blood2006; 108:1388-94.
Graham RM, Chua ACG, Herbison CE, et al. Liver iron transport. World J. Gastroenterol.2007; 13:4725-36.
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