|Application ||WB, E|
|Other Accession||EAW76907, 119597313|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||51981 Da|
|Application Notes||STEAP4 antibody can be used for detection of STEAP4 by Western blot at 0.5 - 1 µg/mL.|
|Target/Specificity||STEAP4; At least two isoforms are known to exist for this protein. This STEAP4 antibody does not cross-react with other STEAP proteins.|
|Reconstitution & Storage||STEAP4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||STEAP4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+) (By similarity). Uses NADP(+) as acceptor (By similarity). Plays a role in systemic metabolic homeostasis, integrating inflammatory and metabolic responses (By similarity). Associated with obesity and insulin- resistance (PubMed:18430367, PubMed:18381574). Involved in inflammatory arthritis, through the regulation of inflammatory cytokines (PubMed:19660107). Inhibits anchorage-independent cell proliferation (PubMed:19787193).|
|Cellular Location||Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein|
|Tissue Location||Ubiquitous. Highly expressed in adipose tissue. Expressed in placenta, lung, heart and prostate. Detected at lower levels in liver, skeletal muscle, pancreas, testis and small intestine. Highly expressed in joints of patients with rheumatoid arthritis and localized with CD68 cells, a marker for macrophages.|
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Provided below are standard protocols that you may find useful for product applications.
STEAP4 Antibody: The six-transmembrane epithelial antigen of prostate 4 (STEAP4) is a member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. Similar to two other members of the STEAP family (STEAP 2 and STEAP3), STEAP4 promotes both iron and copper reduction. STEAP4 is highly expressed in placenta, lung, heart and prostate tissues, and overexpressed in prostate cancer cells compared to normal prostate cells. Overexpression of STEAP4 in prostate cells significantly increases cell growth and colony formation, suggesting STEAP4 may have a role in cell proliferation and prostate cancer progression.
Korkmaz CG, Korkmaz KS, Kurys P, et al. Molecular cloning and characterization of STAMP2, an androgen-regulated six transmembrane protein that is overexpressed in prostate cancer. Oncogene 2005; 24:4934-45.
Ohgami RS, Campagna DR, McDonald A, et al. The Steap proteins are metalloreductases. Blood2006; 108:1388-94.
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