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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   TRIM25 Antibody   

TRIM25 Antibody

  • WB - TRIM25 Antibody ASC10577
    Western blot analysis of TRIM25 in HeLa cell lysate with TRIM25 antibody at (A) 0.5 and (B) 1 µg/mL.
  • ICC - TRIM25 Antibody ASC10577
    Immunocytochemistry of TRIM25 in HeLa cells with TRIM25 antibody at 5 µg/mL.
  • IF - TRIM25 Antibody ASC10577
    Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14258
Other Accession NP_005073, 68160937
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 70973 Da
Application Notes TRIM25 antibody can be used for detection of TRIM25 by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 7706
Target/Specificity TRIM25;
Reconstitution & Storage TRIM25 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsTRIM25 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRIM25
Synonyms EFP, RNF147, ZNF147
Function Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.
Cellular Location Cytoplasm. Note=Colocalized with DDX58 at cytoplasmic perinuclear bodies
Tissue Location Ubiquitous..
Research Areas
Citations (0)

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TRIM25 Antibody: TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.


Inoue S, Orimo A, Hosoi T, et al. Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein. Proc. Natl. Acad. Sci. USA1993; 11117-20.
Orimo A, Inoue S, Minowa O, et al. Underdeveloped uterus and reduced estrogen responsiveness in mice with disruption of the estrogen-responsive finger protein gene, which is a direct target of estrogen receptor a. Proc. Natl. Acad. Sci. USA1999; 96:12027-32.
Thomson SD, Ali S, Pickles L, et al. Analysis of estrogen-responsive finger protein expression in benign and malignant human breast. Int. J. Cancer2001; 91:152-8.
Urano T, Saito T, Tsukui T, et al. Efp targets 14-3-3s for proteolysis and promotes breast tumour growth. Nature2002; 417:871-5.

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$ 310.00
Cat# ASC10577
(40 western blots)
Availability: 5-7days
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