|Application ||WB, E|
|Application Notes||StrepII-tag antibody can be used for the detection of StrepII-tagged proteins by Western blot at 1 µg/mL.|
|Reconstitution & Storage||StrepII-tag antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||StrepII-tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.
Schmidt TGM and Skerra A. The random peptide-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng.1993; 6:109-22.
Schmidt TGM, Koepe J, Frank R, et al. Molecular interactions between the Strep-tag affinity peptide and its cognate target, streptavidin. J. Mol. Biol.1996; 255:753-6.
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