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StrepII-tag Antibody

  • WB - StrepII-tag Antibody ASC10585
    Western blot analysis of (A) 5 and (B) 25ng of StrepII-tagged recombinant protein with StrepII antibody at 1 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application Notes StrepII-tag antibody can be used for the detection of StrepII-tagged proteins by Western blot at 1 µg/mL.
Additional Information
Reconstitution & Storage StrepII-tag antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsStrepII-tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)

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StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.


Schmidt TGM and Skerra A. The random peptide-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng.1993; 6:109-22.
Schmidt TGM, Koepe J, Frank R, et al. Molecular interactions between the Strep-tag affinity peptide and its cognate target, streptavidin. J. Mol. Biol.1996; 255:753-6.

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$ 310.00
Cat# ASC10585
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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