|Application ||WB, IHC-P, IF, E|
|Other Accession||Q96PE2, 9828|
|Calculated MW||221673 Da|
|Application Notes||TEM4 antibody can be used for detection of TEM4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.|
|Target/Specificity||TEM4 antibody was raised against a 18 amino acid synthetic peptide near the center of human TEM4.|
The immunogen is located within amino acids 910 - 960 of TEM4.
|Reconstitution & Storage||TEM4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TEM4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases.|
|Tissue Location||Highly expressed in the heart.|
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Provided below are standard protocols that you may find useful for product applications.
TEM4 Antibody: Rho GTPases, which are activated by specific guanine-nucleotide exchange factors (GEFs), play pivotal roles in several cellular functions. TEM4, encoding a protein containing 1510 amino acids, contains a RhoGEF-specific Dbl homology (DH) domain but lacks their typical pleckstrin homology domain. TEM4 is a Rho-specific GEF with novel structural and regulatory properties and predominant expression in the heart. It couples tyrosine kinase signals with the activation of the rho/rac GTPases, thus leading to cell differentiation and/or proliferation. Elevated levels of TEM4, TEM5, TEM6, TEM7 and TEM7R were also raised in breast cancer tissues. TEM4 could also prove to be useful targets therapeutically.
Ruemenapp U, Freichel-Blomquist A, Wittinghofer B, et al. A mammalian Rho-specific guanine-nucleotide exchange factor (p164-RhoGEF) without a pleckstrin homology domain. Biochem. J.2002; 366:721-8.
Carson-Walter EB, Watkins DN, Nanda A, et al. Cell surface tumor endothelial markers are conserved in mice and humans. Cancer Res.2001; 61:6649-55.
Davies G, Cunnick GH, Mansel RE, et al. Levels of expression of endothelial markers specific to tumour-associated endothelial cells and their correlation with prognosis in patients with breast cancer. Clinical & Experimental Metastasis2004; 21:31-7.
Nanda A and St Croix B. Tumor endothelial markers: new targets for cancer therapy. Curr. Opin. Oncol.2004; 16:44-9.
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