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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   TEM5 Antibody   

TEM5 Antibody

     
  • WB - TEM5 Antibody ASC10601
    Western blot analysis of TEM5 in rat kidney tissue lysate with TEM5 antibody at (A) 2 µg/ml and (B) 4 µg/mL.
  • IHC - TEM5 Antibody ASC10601
    Immunohistochemistry of TEM5 in human bladder tissue with TEM5 antibody at 5 µg/mL.
  • IF - TEM5 Antibody ASC10601
    Immunofluorescence of TEM5 in human bladder tissue with TEM5 antibody at 20 µg/mL.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q96PE1
Other Accession Q96PE1, 221222450
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 142647 Da
Application Notes TEM5 antibody can be used for detection of TEM5 by Western blot at 2 and 4 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 25960
Target/Specificity GPR124;
Reconstitution & Storage TEM5 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsTEM5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADGRA2 (HGNC:17849)
Function Endothelial receptor which functions as a WNT7-specific coactivator of canonical Wnt signaling (By similarity). Required for normal endothelial cell sprouting and migration in the forebrain and neural tube (By similarity). Has a major role in establishing the blood-brain barrier (By similarity). Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate (PubMed:16982628).
Cellular Location Cell membrane; Multi- pass membrane protein. Cell projection, filopodium. Note=Enriched at lateral cell borders and also at sites of cell-ECM (extracellular matrix) contact.
Tissue Location Expressed in endothelial cells (at protein level) (PubMed:15021905, PubMed:16982628). Abundantly expressed in heart, placenta, ovary, small intestine, and colon (PubMed:15021905).
Research Areas
Citations (0)

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Background

TEM5 Antibody: Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogensis that are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. These include TEMs such as TEM1, TEM5, TEM7 and TEM8. TEM5 is a member of the adhesion family of G protein coupled receptors and is localized on the surface of endothelial cells. TEM5 is a seven-pass transmembrane receptor, unlike TEM1, TEM7 and TEM8 which span the membrane once. TEM5 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.

References

Carson-Walter EB, Watkins DN, Nanda A, et al. Cell surface tumor endothelial markers are conserved in mice and humans. Cancer Res.2001; 61:6649-55.
Yamamoto Y, Irie K, Nanda A, et al. Direct binding of the human homologue of the Drosophila disc large tumor suppressor gene to seven-pass transmembrane proteins, tumor endothelial marker 5 (TEM5), and a novel TEM5-like protein. Oncogene2004; 23:3889-97.
Davies G, Cunnick GH, Mansel RE, et al. Levels of expression of endothelial markers specific to tumour-associated endothelial cells and their correlation with prognosis in patients with breast cancer. Clinical & Experimental Metastasis2004; 21:31-7.
Vallon M and Essler M. Proteolytically processed soluble tumor endothelial marker (TEM) 5 mediates endothelial cell survival during angiogenesis by linking integrin alpha(v)beta3 to glycosaminoglycans. J. Biol. Chem.2006; 281:34179-88.

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$ 320.00
Cat# ASC10601
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