|Application ||WB, IF, ICC, E|
|Other Accession||P08620, 122750|
|Calculated MW||22048 Da|
|Application Notes||FGF4 antibody can be used for detection of FGF4 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 µg/mL. For immunofluorescence start at 2.5 µg/mL.|
|Reconstitution & Storage||FGF4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||FGF4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||HST, HSTF1, KS3|
|Function||Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal limb and cardiac valve development during embryogenesis.|
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Provided below are standard protocols that you may find useful for product applications.
FGF4 Antibody: Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.
Powers CJ, McLeskey SW, and Wellstein A. Fibroblast growth factors, their receptors and signaling. Endocr. Relat. Cancer2000; 7:165-97.
Delli-Bovi P, Curatola AM, Kern FG, et al. An oncogene isolated by transfection of Kaposi's sarcoma DNA encodes a growth factor that is a member of the FGF family. Cell1987; 50:729-37.
Yoshida T, Ishimaru K, Sakamoto H, et al. Angiogenic activity of the recombinant hst-1 protein. Cancer Lett.1994; 83:261-268.
Laufer E, Nelson CE, Johnson RL, et al. Sonic hedgehog and Fgf-4 act through a signaling cascade and feedback loop to integrate growth and patterning of the developing limb bud. Cell1994; 79:993-1003.
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