|Application ||WB, IHC-P, IF, E|
|Other Accession||Q96NN9, 74732608|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||66791 Da|
|Application Notes||AIFM3 antibody can be used for the detection of AIFM3 by Western blot at 0.5 and 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||AIFM3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||AIFM3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Induces apoptosis through a caspase dependent pathway. Reduces mitochondrial membrane potential.|
|Cellular Location||Mitochondrion. Note=Does not translocate to the nucleus upon induction of apoptosis|
|Tissue Location||Ubiquitous. Expressed in bone marrow, cerebral cortex, liver, ovary, thymus, thyroid gland and tongue (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
AIFM3 Antibody: Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. Recently a protein similar to the apoptosis-inducing factor (AIF) was cloned and designated AIFL (also known as AIFM3). AIFM3 is expressed ubiquitously and is predominantly localized to the inner membranes of mitochondria. Unlike AIF, AIFM3 does not translocate to the nucleus upon induction of apoptosis. However, overexpression of AIFM3, like AIF, induced cytochrome c release from the mitochondria, cleavage of caspase 3, and ultimately apoptosis, indicating AIFM3 induces apoptosis through caspase activation. Multiple isoforms of AIFM3 are known to exist.
Jin Z and El Deiry WS. Overview of cell death signaling pathways. Cancer Biol. Ther.2004; 4:139-63
Xie Q, Lin T, Zhang Y, et al. Molecular cloning and characterization of a human AIF-like gene with the ability to induce apoptosis. J. Biol. Chem.2005; 280:19673-81.
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