|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_060424, 148839294|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 138, 145 kDa |
Observed: 150 kDa
|Application Notes||CAPS2 antibody can be used for detection of CAPS2 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||CADPS2; Multiple isoforms of CAPS2 are known to exist. This CAPS2 antibody is predicted to be specific to CAPS2 and not recognize CAPS1.|
|Reconstitution & Storage||CAPS2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||CAPS2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Calcium-binding protein involved in exocytosis of vesicles filled with neurotransmitters and neuropeptides. Probably acts upstream of fusion in the biogenesis or maintenance of mature secretory vesicles. Regulates neurotrophin release from granule cells leading to regulate cell differentiation and survival during cerebellar development. May specifically mediate the Ca(2+)- dependent exocytosis of large dense-core vesicles (DCVs) and other dense-core vesicles (By similarity).|
|Cellular Location||Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Note=Membrane-associated to vesicles. Strongly enriched in synaptic fractions. Probably localizes to different vesicles compared to CADPS. Enriched on vesicular structures in the parallel fiber terminal of granule cells that are distinct from synaptic vesicles|
|Tissue Location||Widely expressed. Expressed in all adult and fetal tissues examined, with the strongest expression in kidney and pancreas. In brain, it is expressed at high levels in cerebellum, to a lesser degree in cerebral cortex, occipital pole, and frontal and temporal lobes. Only weakly expressed in medulla, spinal cord and putamen.|
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Provided below are standard protocols that you may find useful for product applications.
CAPS2 Antibody: CAPS2 and the related protein CAPS1 encode novel neural/endocrine-specific cytosolic and peripheral membrane proteins. Both are essential components of the synaptic vesicle priming machinery and are required for the Ca2+-regulated exocytosis of secretory vesicles; CAPS-deficient neurons contain no or very few fusion competent synaptic vesicles, causing a selective impairment of fast phasic transmitter release. Like CAPS1, numerous isoforms of CAPS2 are known to exist. These isoforms show distinct expression patterns in the brain. For example, CAPS2b showed high expression in the developing cerebellum. Furthermore, one version of CAPS2 mRNA that lacks exon 3 is detected in some autistic patients, suggesting that the differential expression pattern of CAPS2 is involved in neuronal development.
Walent JH, Porter BW, and Martin TF. A novel 145 kD brain cytosolic protein reconstitutes Ca(2+)-regulated secretion in permeable neuroendocrine cells. Cell 1992; 70:765-775.
Juckusch WJ, Speidel D, Sigler A, et al. CAPS-1 and CAPS-2 are essential synaptic vesicle priming proteins. Cell 2007; 131:796-808.
Sadakata T, Washida M, and Furuichi T. Alternative splicing variants in mouse CAPS2: differential expression and functional properties of splicing variants. BMC Neurosci. 2007; 8:25-35.
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