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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   VPS53 Antibody   

VPS53 Antibody

  • WB - VPS53 Antibody ASC10687
    Western blot analysis of VPS53 in 293 cell lysate with VPS53 antibody at (A) 0.5 and (B) 1 µg/mL.
  • ICC - VPS53 Antibody ASC10687
    Immunocytochemistry of VPS53 in 293 cells with VPS53 antibody at 5 µg/mL.
  • IF - VPS53 Antibody ASC10687
    Immunofluorescence of VPS53 in 293 cells with VPS53 antibody at 2.5 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q5VIR6
Other Accession EAW90658, 119611064
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 79653 Da
Application Notes VPS53 antibody can be used for detection of VPS53 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 2.5 µg/mL.
Additional Information
Gene ID 55275
Target/Specificity VPS53;
Reconstitution & Storage VPS53 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsVPS53 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VPS53
Function May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.
Cellular Location Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein
Research Areas
Citations (0)

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VPS53 Antibody: The sorting of acid hydrolases to lysosomes rely on mannose 6-phosphate receptors that cycle between the trans-Golgi network (TGN) and endosomes. The maintenance of this cycle requires the function of the mammalian Golgi-associated retrograde protein (GARP) complex which is composed of three subunits: VPS52, VPS53, and VPS54. Depletion of any of these three proteins, such as by RNAi, impairs the retrograde transport of multiple TGN proteins. VPS53 was identified as an HIV dependency factor (HDF) and plays a role in viral entry to the cell, suggesting that VPS53 may be an important drug target in HIV treatment. At least five isoforms of VPS53 are known to exist.


Pfeffer SR. Targeting of proteins to the lysosome. Curr. Top. Microbiol. Immunol.1991; 170:43-65.
Liewen H, Meinhold-Heerlein I, Oliveira V, et al. Characterization of the human GARP (Golgi associated retrograde protein) complex. Exp. Cell Res.2005; 306:22-34.
Perez-Victoria FJ, Mardones GA, and Bonifacino JS. Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes. Mol. Biol. Cell2008; 19:2350-62.
Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.

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$ 310.00
Cat# ASC10687
(40 western blots)
Availability: 5-7days
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