|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_003369, 17136078|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 68 kDa |
Observed: 80 kDa
|Application Notes||VGF antibody can be used for detection of VGF by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||VGF antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||VGF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system. Antimicrobial peptide VGF[554-577]: Has bactericidal activity against M. luteus, and antifungal activity against P. Pastoris.|
|Cellular Location||Secreted. Cytoplasmic vesicle, secretory vesicle. Note=Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus|
|Tissue Location||Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells|
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Provided below are standard protocols that you may find useful for product applications.
VGF Antibody: VGF was initially identified as a rapidly regulated gene product in nerve growth factor-treated PC12 cells. This protein is synthesized in neurons in the central and peripheral nervous system as well as in the pituitary, adrenal medulla, endocrine cells of the stomach, and pancreatic beta cells. VGF is thought to be involved in organism energy balance and regulation of homeostasis as mice lacking this gene show deficits in these areas. More recent results suggest that VGF is upregulated by brain-derived neurotrophic factor (BDNF) and can stimulate the proliferation of hippocampal progenitor cells and produce antidepressant-like behavioral effects, suggesting that this pathway may be a suitable target for therapeutic treatments.
Levi A, Eldridge JD, and Paterson BM. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 1985; 229:393-5.
Possenti R, Eldridge JD, Paterson BM, et al. A protein induced by NGF in PC12 cells is stored in secretory vesicles and released through the regulatory pathway. EMBO J.1989; 8:2217-23.
Hahm S, Mizuno TM, Wu TJ, et al. Targeted deletion of the Vgf gene indicates that the encoded secretory peptide precursor plays a novel role in the regulation of energy balance. Neuron 1999; 23:537-48.
Thakker-Varia S, Krol JJ, Nettleton J, et al. The neuropeptide VGF produces antidepressant-like behavioral effects and enhances proliferation in the hippocampus. J. Neurosci. 2007; 27:12156-67.
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