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Wnt10b Antibody

  • WB - Wnt10b Antibody ASC10699
    Western blot analysis of Wnt10b in human skeletal muscle tissue lysate with Wnt10b antibody at (A) 2 and (B) 4 µg/mL.
  • IHC - Wnt10b Antibody ASC10699
    Immunohistochemistry of Wnt10b in human skeletal muscle with Wnt10b antibody at 2.5 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00744
Other Accession NP_003385, 16936522
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 43 kDa

Observed: 47 kDa
Application Notes Wnt10b antibody can be used for detection of Wnt10b by Western blot at 2 - 4 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL.
Additional Information
Gene ID 7480
Target/Specificity WNT10B; Two isoforms of Wnt10b are known to exist; this antibody will only recognize the longer isoform. This Wnt10b antibody will not cross-react with Wnt10a.
Reconstitution & Storage Wnt10b antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsWnt10b Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WNT10B
Synonyms WNT12
Function Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Detected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain
Research Areas
Citations (0)

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Wnt10b Antibody: Wnt10b is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Wnt10b is found in the mouse mammary tumor virus insertion site where it is activated and causes mammary tumors. Elevated levels of Wnt10b have also been detected in human breast carcinomas. Wnt10b is known to be involved in adipogenesis, maintaining the preadipocyte in an undifferentiated state. More recently, Wnt10b has been shown to promote epithelial cell differentiation and hair shaft growth, demonstrating that Wnt10b has multiple roles in cell growth and differentiation.


Lee FS, Lane TF, Kuo A, et al. Insertional mutagenesis identifies a member of the Wnt gene family as a candidate oncogene in the mammary epithelium of int-2/Fgf-3 transgenic mice. Proc. Natl. Acad. Sci. USA1995; 92:2268-72.
Nusse R and Varmus RE. Wnt genes. Cell1992; 69:1073-87.
Bui TD, Rankin J, Smith K, et al. A novel human Wnt gene, WNT10B, maps to 12q13 and is expressed in human breast carcinomas. Oncogene1997; 14:1249-53.
Bennett CN, Hodge CL, MacDougald OA, et al. Role of Wnt10b and C/EBPalpha in spontaneous adipogenesis of 243 cells. Biochem. Biophys. Res. Commun.2003; 302:12-16.

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$ 310.00
Cat# ASC10699
(40 western blots)
Availability: 5-7days
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