|Application ||WB, IHC-P, IF, E|
|Other Accession||O15079, 21362912|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||53537 Da|
|Application Notes||Syntaphilin antibody can be used for detection of Syntaphilin by Western blot at 1 - 2 µg/mL. Despite its predicted molecular weight, Syntaphilin usually migrates at higher molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||Syntaphilin antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Syntaphilin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits SNARE complex formation by absorbing free syntaxin-1.|
|Cellular Location||Membrane; Single-pass membrane protein. Cell junction, synapse, synaptosome|
|Tissue Location||Brain specific. Found in synapses.|
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Provided below are standard protocols that you may find useful for product applications.
Syntaphilin Antibody: Syntaphilin was initially identified in a yeast two-hybrid screen with the carboxy terminal region of Syntaxin-1 as bait. Syntaxin-1 is a key component of the synaptic vesicle docking machinery that forms the SNARE complex with synaptobrevin and SNAP-25. Syntaphilin competes with SNAP-25 for binding to syntaxin-1 and inhibits the formation of the SNARE complex, thereby potentially regulating synaptic vesicle exocytosis. Syntaphilin also binds dynamin-1 and inhibits dynamin-dependent endocytosis. Mice lacking syntaphilin show an increased level of mitochondrial motility and a reduced density of axonal mitochondria. This correlates with an enhanced short-term facilitation and significant impairments in motor ability, suggesting syntaphilin plays a major role in presynaptic function. Multiple isoforms are known to exist.
Lao G, Scheuss V, Gerwin CM, et al. Syntaphilin: a syntaxin-1 clamp that controls SNARE assembly. Neuron2000; 25:191-201.
Sorensen JB. SNARE complexes prepare for membrane fusion. Trends Neurosci.2005; 28:453-5.
Das S, Gerwin C, and Sheng ZH. Syntaphilin binds to dynamin-1 and inhibits dynamin-dependent endocytosis. J. Biol. Chem.2003; 278:41221-6.
Kang J-S, Tian J-H, Pan P-Y, et al. Docking of axonal mitochondria by syntaphilin controls their mobility and affects short-term facilitation. Cell2008; 132:137-148.
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