|Application ||WB, IHC-P, IF, E|
|Other Accession||Q9GZT5, 14424011|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 46 kDa |
Observed: 46 kDa
|Application Notes||Wnt10a antibody can be used for detection of Wnt10a by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||WNT10A; This Wnt10a antibody is predicted to not cross-react with Wnt10b.|
|Reconstitution & Storage||Wnt10a antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Wnt10a Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.|
|Cellular Location||Secreted, extracellular space, extracellular matrix|
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Provided below are standard protocols that you may find useful for product applications.
Wnt10a Antibody: Wnt10a is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Elevated levels of Wnt10a have been detected in several human tumors and cancer cell lines including colorectal cancer and chronic lymphocytic leukemia and it has been suggested that uncontrolled Wnt signaling may contribute to the defect in apoptosis that characterizes these malignancies. Recently, a mutation in Wnt10a was found to be associated with odonto-onycho-dermal dysplasia, an autosomal recessive ectodermal dysplasia.
Kelly GM, Lai CJ, and Moon RT. Expression of wnt10a in the central nervous system of developing zebrafish. Dev. Biol. 1993; 158:113-21.
Nusse R and Varmus RE. Wnt genes. Cell 1992; 69:1073-87.
Kirikoshi H, Sekihara H, and Katoh M. WNT10A and WNT6, clustered in human chromosome 2q35 region head-to-tail manner, are strongly coexpressed in SW480 cells. Biochem. Biophys. Res. Commun. 2001; 283:798-805.
Kirikoshi H, Inoue S, Sekihara H, et al. Expression of WNT10A in human cancer. Int. J. Oncol. 2001; 19:997-1001.
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