|Application ||WB, IHC-P, IF, E|
|Other Accession||P60321, 41688561|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||15132 Da|
|Application Notes||Nanos2 antibody can be used for detection of Nanos2 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||Nanos2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Nanos2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state (By similarity).|
|Cellular Location||Cytoplasm. Cytoplasm, P-body. Cytoplasm, perinuclear region. Note=Localizes at P-bodies during gonocyte development (By similarity). More abundant in perinuclear region of the cytoplasm of the germ cells of the adult testis|
|Tissue Location||Testis and ovary. Expression found in several spermatogenic stages: in cells on the periphery of the tubules which could correspond to spermatogonia, in spermatocytes and in round spermatids (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
Nanos2 Antibody: Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects. This Nanos2 antibody will not cross-react with either Nanos 1 or Nanos3.
Lehmann R and Nusslein-Volhard C. The maternal gene nanos has a central role in posterior pattern formation of the drosophila embryo. Development1991; 112:679-91.
Tsuda M, Sasaoka Y, Kiso M, et al. Conserved role of nanos proteins in germ cell development. Science2003; 301:1239-41.
Sato K, Hayashi Y, Ninomiya Y, et al. Maternal Nanos represses hid/skl-dependent apoptosis to maintain the germ line in Drosophila embryos. Proc. Natl. Acad. Sci. USA2007; 104:7455-60.
Haraguchi S, Tsuda M, Kitajima S, et al. Nanos1: a mouse nanos gene expressed in the central nervous system is dispensable for normal development. Mech. Dev.2003; 120:721-31.
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