|Application ||WB, E|
|Other Accession||NP_055954, 14028875|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||39240 Da|
|Application Notes||Slc35D1 antibody can be used for detection of Slc35D1 by Western blot at 1 - 2 µg/mL.|
|Target/Specificity||SLC35D1; This antibody is predicted to not cross-react with the highly homologous Slc35D2.|
|Reconstitution & Storage||Slc35D1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Slc35D1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transports both UDP-glucuronic acid (UDP-GlcA) and UDP- N-acetylgalactosamine (UDP-GalNAc) from the cytoplasm into the endoplasmic reticulum lumen (PubMed:11322953, PubMed:17952091). Plays a role in chondroitin sulfate biosynthesis, which is important for formation of cartilage extracellular matrix and normal skeletal development (By similarity).|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
Slc35D1 Antibody: The solute carrier family Slc35 consists of at least 17 proteins that act as nucleotide sugar transporters localized to the Golgi apparatus and endoplasmic reticulum. The role of the ER-resident Slc family member Slc35D1 is to transport both UDP-glucuronic acid and UDP-N-acetylgalactosamine. These molecules can serve as substrates for chondroitin sulfate biosynthesis and mice lacking the Slc35D1 gene developed a lethal form of skeletal dysplasia with severe shortening of limbs and facial structures. Examination of epiphyseal cartilage in these mice revealed a decreased proliferating zone with round chrondrocytes, scarce matrices, and reduced proteoglycan aggregates. Loss of function mutations in human Slc35D1 cause Schneckenbecken dysplasia, a severe skeletal dysplasia.
Ishida N and Kawakita M. Molecular physiology and pathology of the nucleotide sugar transporter family (SLC35). Pflugers Arch.2004; 447:768-75.
Muraoka M, Kawakita M, and Ishita N. Molecular characterization of human UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter, a novel nucleotide sugar transporter with dual substrate specificity. FEBS Lett.2001; 495:87-93.
Hiraoka S, Furuichi T, Nishimura G, et al. Nucleotide-sugar transporter Slc35D1 is critical to chondroitin sulfate synthesis in cartilage and skeletal development in mouse and human. Nat. Med.2007; 13:1363-7.
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