|Application ||WB, IF, ICC, E|
|Other Accession||NP_001705, 51039802|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||110750 Da|
|Application Notes||BICD1 antibody can be used for detection of BICD1 by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 µg/mL. For immunofluorescence start at 10 µg/mL.|
|Target/Specificity||BICD1; At least two isoforms of BICD1 are known to exist; this antibody will only recognize the larger isoform.|
|Reconstitution & Storage||BICD1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||BICD1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulates coat complex coatomer protein I (COPI)- independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex.|
|Cellular Location||Golgi apparatus.|
|Tissue Location||Expressed in the brain, heart and skeletal muscle|
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Provided below are standard protocols that you may find useful for product applications.
BICD1 Antibody: BICD1 was initially identified as the human homolog to Drosophila Bicaudal-D protein that forms part of the cytoskeleton and mediates the correct sorting of mRNAs for oocyte- and axis-determining factors during oogenesis. BICD1 can bind to dynein-dynactin and co-localizes with Rab6A on the trans-Golgi network and cytoplasmic vesicles, suggesting BICD1 regulates COPI-independent Golgi-ER transport. BICD1 will also interact with Rab6B, a Rab6 isoform that is expressed primarily in brain. Live cell imaging showed bi-directional movement of Rab6B structures in SK-N-SH neurites, indicating that BICD1 is involved in the Rab6B regulation of retrograde cargo transport in neuronal cells.
Baens M and Marynen P. A human homologue (BICD1) of the drosophila bicaudal-D gene. Genomics1997; 45:601-6.
Matanis T, Akhmanova A, Wulf P, et al. Bicaudal-D regulates COPI-independent Golgi-ER transport by recruiting the dynein-dynactin motor complex. Nat. Cell Biol.2002; 986-92.
Wanshers BF, van de Vorstenbosch R, Schlager MA, et al. A role for the Rab6B Bicaudal-D1 interaction in retrograde transport in neuronal cells. Exp. Cell Res.2007; 313:3408-20.
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