|Application ||WB, E|
|Other Accession||NP_006109, 13435356|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||31621 Da|
|Application Notes||Hax1a antibody can be used for detection of Hax1A by Western blot at 1 - 2 µg/mL.|
|Target/Specificity||HAX1; At least four isoforms of Hax1 are known to exist. This antibody is expected to recognize the longest isoform (Hax1a) as well as the shortest.|
|Reconstitution & Storage||Hax1a antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Hax1a Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Promotes cell survival. Potentiates GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. May regulate intracellular calcium pools.|
|Cellular Location||Mitochondrion. Endoplasmic reticulum. Nucleus membrane. Cytoplasmic vesicle. Sarcoplasmic reticulum|
|Tissue Location||Ubiquitous. Up-regulated in oral cancers.|
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Provided below are standard protocols that you may find useful for product applications.
Hax1a Antibody: The HS-1 associated protein X-1 (Hax1) was initially identified in a yeast two-hybrid assay on the basis of its ability to bind to the hemapoietic cell-specific protein 1 (HS-1). Hax1 possesses anti-apoptotic activity and is structurally related to Bcl-2 family members, including the presence of BH1- and BH2-like domains. It has recently been shown to interact with HIV viral protein R (Vpr), a protein required for viral pathogenesis of HIV and linked to T-cell apoptosis through activation of caspases 3 and 9. Other studies indicate that Hax1-mediated processing of HtrA2 (also known as Omi) by the mitochondrial protease PARL allows survival of lymphocytes and neurons when cytokines are limiting.
Suzuki Y, Demoliere C, Kitamura D, et al. HAX-1, a novel intracellular protein, localized on mitochondria directly associates with HS1, a substrate of Src family tyrosine kinases. J. Immunol.1997; 158:2736-44.
Sharp TV, Wang HW, Koumi A, et al. K15 protein of Kaposi’s sarcoma-associated herpesvirus is latently expressed and binds to HAX-1, a protein with antiapoptotic function. J. Virol.2002; 76:802-16.
Yedavalli VS, Shih HM, Chiang YP, et al. Human immunodeficiency virus type 1 Vpr interacts with antiapoptotic mitochondrial protein HAX-1. J. Virol.2005; 79:13735-46.
Chao J-R, Parganas E, Boyd K, et al. Hax1-mediated processing of HtrA2 by Parl allows survival of lymphocytes and neurons. Nature2008; 452:98-102.
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