|Other Accession||P89079, 62510674|
|Calculated MW||14041 Da|
|Application Notes||Ad-9 E4 Orf1 antibody can detect 10ng Ad-9 E4 Orf1 peptide in ELISA at 1 µg/mL.|
|Reconstitution & Storage||Adenovirus-9 E4 Orf1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Adenovirus-9 E4 Orf1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a key role in virus oncogenecity in animals. Binds and sequesters human MUPP1/MPDZ protein in the cytoplasm, preventing it from playing a role in cellular proliferation regulation. Induces cell transformation, probably by inactivating MPDZ protein.|
|Cellular Location||Host cytoplasm.|
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Provided below are standard protocols that you may find useful for product applications.
Adenovirus-9 E4 Orf1 Antibody: The many different serotypes of human adenoviruses (Ad) are divided into six subgroups, of which all Ad subgroup A and B and two subgroup D Ads can elicit tumors in infected rodents. Unlike the Ads from subgroup A and B, the ones from subgroup D, Ad9 and Ad10 elicit estrogen-dependent mammary tumors as opposed to undifferentiated sarcomas. In the case of Ad9, its tumorigenicity is dependent on the product of the open reading frame (ORF) 1 of the early region 4 (E4). The tumorigenic potential of Ad9 E4 Orf1 depends on a carboxyl-terminal PDZ domain-binding motif that mediates interactions with several different membrane-associated cellular proteins such as MUPP1, PATJ, MAGI-1, ZO-2 and Dlg1. It has been suggested that Ad9 E4 Orf1 may have evolved from an ancestral cellular dUTP pyrophosphatase.
Shenk T. Adenoviridae: the viruses and their replication, p. 2265-2300 in DM Knipe and PM Howley (ed.) Fields virology, 4th ed.Vol. 2. 2001. Lippincott-Raven Publishers, Philadelphia, PA.
Javier R, Rasha Jr. K, Macdonald GJ, et al. Human adenovirus type 9-induced rat mammary tumors. J. Virol.1991; 65:3192-202.
Javier RT. Adenovirus type 9 E4 open reading frame 1 encodes a transforming protein required for the production of mammary tumors in rats. J. Virol.1994; 68:3917-24.
Weiss RS, Lee SS, Prasad BV, et al. Human adenovirus early region 4 open reading frame 1 genes encode growth-transforming proteins that may be distantly related to dUTP pyrophosphatase enzymes. 1997; J. Virol.1997; 71:1857-70.
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