|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_037481, 7019545|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||18856 Da|
|Application Notes||NENF antibody can be used for detection of NENF by Western blot at 1 - 2 µg/mL. Despite its predicted molecular weight of ~20kDa, NENF is observed at 37 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||NENF antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||NENF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival. Promotes cell proliferation and neurogenesis in undifferentiated neural pro-genitor cells at the embryonic stage and inhibits differentiation of astrocyte. Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways. Neurotrophic activity is enhanced by binding to heme. Acts also as an anorexigenic neurotrophic factor that contributes to energy balance (By similarity). Plays a role in the human tumorigenesis (PubMed:22748190).|
|Cellular Location||Secreted, extracellular space.|
|Tissue Location||Ubiquitously expressed with high expression in heart. Over-expressed in various tumors including carcinomas of the uterine cervix, lymphoma, colon, lung, skin and leukemia, as well as carcinoma of the breast.|
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Provided below are standard protocols that you may find useful for product applications.
NENF Antibody: NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor.
Kimura I, Yoshioka M, Konishi M, et al. Neudesin, a novel secreted protein with a unique primary structure and neurotrophic activity. J. Neurosci. Res.2005; 79:287-94.
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