|Application ||WB, E|
|Other Accession||NP_064585, 9910262|
|Calculated MW||25674 Da|
|Application Notes||PRTFDC1 antibody can be used for detection of PRTFDC1 by Western blot at 1 µg/mL.|
|Reconstitution & Storage||PRTFDC1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||PRTFDC1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has low, barely detectable phosphoribosyltransferase activity (in vitro). Binds GMP, IMP and alpha-D-5-phosphoribosyl 1-pyrophosphate (PRPP). Is not expected to contribute to purine metabolism or GMP salvage.|
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Provided below are standard protocols that you may find useful for product applications.
PRTFDC1 Antibody: Phosphoribosyl transferase domain containing 1 (PRTFDC1) is highly homologous to the hypoxanthine phosphoribosyltransferase (HPRT1) and may have arisen from a gene duplication event of a common ancestor gene. Recently, it was shown that CpG islands in the PRTFDC1 promoter could be hypermethylated in ovarian cancers and oral squamous-cell carcinomas (OSCC), leading to gene silencing. Restoration of PRTFDC1 expression in OSCC inhibited cell growth in colony-formation assays, while knockdown of PRTFDC1 expression in OSCC that expressed the gene promoted cell growth. These results suggest that PRTFDC1 can act as a tumor-suppressor gene. At least three isoforms of PRTFDC1 are known to exist.
Nicklas JA. Pseudogenes of the human HPRT1 gene. Environ. Mol. Mutagen2006; 47:212-8.
Keebaugh AC, Sullivan RT, NISC Comparative Sequencing Program, et al. Gene duplication and inactivation in the HPRT gene family. Genomics2007; 89:134-42.
Cai LY, Abe M, Izumi S, et al. Identification of PRTFDC1 silencing and aberrant promoter methylation of GPR150, ITGA8 and HOXD11 in ovarian cancers. Life Sci.2007; 80:1458-65.
Suzuki E, Imoto I, Pimkhaokham A, et al. PRTFDC1, a possible tumor-suppressor gene, is frequently silenced in oral squamous-cell carcinoma by aberrant promoter hypermethylation. Oncogene2007; 26:7921-32.
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