|Application ||WB, E|
|Other Accession||NP_037448, 56550057|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||63510 Da|
|Application Notes||CNOT4 antibody can be used for detection of CNOT4 by Western blot at 1 - 2 µg/mL.|
|Reconstitution & Storage||CNOT4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||CNOT4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins (PubMed:11823428, PubMed:22159038, PubMed:26575292). Involved in activation of the JAK/STAT pathway (PubMed:11823428, PubMed:22159038). Catalyzes ubiquitination of methylated RBM15 (PubMed:26575292).|
|Cellular Location||Cytoplasm. Nucleus|
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Provided below are standard protocols that you may find useful for product applications.
CNOT4 Antibody: CNOT4 is a component of the CCR4-NOT transcription complex, a complex that is implicated in the repression of RNA polymerase II transcription. In the CCR4-NOT complex, CNOT4 acts as an E3 ubiquitin-protein ligase and interacts with a subset of E2 ubiquitin-conjugating enzymes through a unique C4C4 RING domain. This E3 ligase activity was shown to be dependent on the selective and specific interaction with the ubiquitin conjugating enzyme UbcH5B. In yeast, mutations in CNOT4 that prevented its interaction with the UbcH5B homolog UBC4 caused increased sensitivity to hydroxyurea, heat shock, and hygromycin B, suggesting that CNOT4 and UbcH5B are involved in stress response in vivo. Multiple isoforms of CNOT4 are known to exist.
Albert TK, Hanzawa H, Legtenberg IA, et al. Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex. EMBO J.2002; 21:355-64.
Winkler GS, Albert TK, Dominguez C, et al. An altered specificity ubiquitin-conjugating enzyme/ubiquitin-protein ligase pair. J. Mol. Biol.2004; 337:157-65.
Mulder KW, Inagaki A, Cameroni E, et al. Modulation of Ubc4p/Ubc5p-mediated stress responses by the RING-finger-dependent ubiquitin-protein ligase Not4p in Saccharomyces cerevisiae. Genetics2007; 176:181-92.
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