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HINT1 Antibody

  • WB - HINT1 Antibody ASC10773
    Western blot analysis of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 µg/mL.
  • ICC - HINT1 Antibody ASC10773
    Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P49773
Other Accession NP_005331, 4885413
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 13802 Da
Application Notes HINT1 antibody can be used for detection of HINT1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 µg/mL.
Additional Information
Gene ID 3094
Target/Specificity HINT1;
Reconstitution & Storage HINT1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsHINT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HINT1
Function Hydrolyzes purine nucleotide phosphoramidates with a single phosphate group, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His- AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O- phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin- protein ligase complex.
Cellular Location Cytoplasm. Nucleus. Note=Interaction with CDK7 leads to a more nuclear localization
Tissue Location Widely expressed.
Research Areas
Citations (0)

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HINT1 Antibody: Histidine triad nucleotide-binding protein 1 (HINT1) is a member of the histidine triad (HIT) protein family, a group of small nucleotide-binding and -hydrolyzing proteins. HINT1 interacts with several diverse proteins and has been suggested to have tumor suppressive activities. HINT1 catalyzes the hydrolysis of adenosine 5'-monophoramidate substrates such as AMP-morpholidate, but its enzymatic function does not appear to play a role in its tumor suppression. Recent experiments demonstrate that HINT1 forms a complex with POSH and JNK in vivo, inhibiting AP-1 activity and the phosphorylation of c-Jun, and this action could contribute to the tumor suppressor activity of HINT1. Other studies raise the possibility of HINT1 as a candidate gene for schizophrenia.


Lima CD, Klein MG, Weinstein IB, et al. Three-dimensional structure of human protein kinase C interacting protein 1, a member of the HIT family of proteins. Proc. Natl. Acad. Sci. USA1996; 93:5357-62.
Weiske J and Huber O. Beta-catenin takes a HIT. Cell Cycle2008; 7:1326-31.
Wang L, Zhang Y, Li H, et al. Hint1 inhibits growth and activator protein-1 activity in human colon cancer cells. Cancer Res.2007; 67:4700-8.
Chen Q, Wang X, O’Neill FA, et al. Is the histidine triad nucleotide-binding protein 1 (HINT1) gene a candidate for schizophrenia? Schizophr. Res.2008; 106:200-7.

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$ 310.00
Cat# ASC10773
(40 western blots)
Availability: 5-7days
Bulk Size
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