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ZBRK1 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9GZX5 |
| Other Accession | NP_067645, 59710107 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 60011 Da |
| Application Notes | ZBRK1 antibody can be used for detection of ZBRK1 by Western blot at 1 µg/mL. |
| Gene ID | 59348 |
|---|---|
| Target/Specificity | ZNF350; |
| Reconstitution & Storage | ZBRK1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | ZBRK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ZNF350 |
|---|---|
| Synonyms | ZBRK1 |
| Function | Transcriptional repressor. Binds to a specific sequence, 5'- GGGxxxCAGxxxTTT-3', within GADD45 intron 3. |
| Cellular Location | Nucleus. Nucleus matrix. Note=Associated with the nuclear matrix |
| Tissue Location | Widely expressed.. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
ZBRK1 Antibody: ZBRK1 is a member of the Kruppel-associated box-zinc finger protein (KRAB-ZFP) protein family of transcriptional repressors. ZBRK1 has been shown to not only be up-regulated in senescent fibroblasts but also interacts with BRCA1, a transcriptional regulator with tumor suppressor activity. ZBRK1 binds to a specific sequence within the GADD45 gene, supporting the assembly of a nuclear complex containing BRCA1 and repressing its transcription. Recent experiments demonstrate that ZBRK1 can homo-oligomerize in vitro and in vivo through a C-terminal transcriptional repression domain (CTRD), and this oligomerization facilitates the ZBRK1-directed transcriptional repression through ZBRK1 response elements. These results suggest that higher order ZBRK1 oligomers may assemble through both protein-DNA and CTRD-dependent protein-protein interactions.
References
Zheng L, Pan H, Li S, et al. Sequence-specific transcription corepressor function for BRCA1 through a novel Zinc finger protein, ZBRK1. Mol. Cell2000; 6:757-68.
Collins T, Stone JR, and Williams AJ. All in the family: the BTB/POZ, KRAB, and SCAN domains. Mol. Cell. Biol.2001; 21:3609-15.
Tan W, Kim S, and Boyer TG. Tetrameric oligomerization mediates transcriptional repression by the BRCA1-dependent Kruppel-associated Box-Zinc finger protein ZBRK1. J. Biol. Chem.2004; 279:55153-60.
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