|Application ||WB, ICC, E|
|Other Accession||NP_076945, 148596996|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 32 kDa |
Observed: 34 kDa
|Application Notes||CUEDC2 antibody can be used for detection of CUEDC2 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 5 µg/mL.|
|Target/Specificity||CUEDC2; CUEDC2 antibody will not cross-react with CUEDC1.|
|Reconstitution & Storage||CUEDC2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||CUEDC2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Down-regulates ESR1 protein levels through the ubiquitination-proteasome pathway, regardless of the presence of 17 beta-estradiol. Also involved in 17 beta-estradiol-induced ESR1 degradation. Controls PGR protein levels through a similar mechanism.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Significantly up-regulated in breast tumor tissues compared with matched adjacent normal tissues (at protein level). Levels inversely correlate with ESR1 in breast cancers and are lower in low-grade tumors compared to high-grade tumors|
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Provided below are standard protocols that you may find useful for product applications.
CUEDC2 Antibody: The CUE (coupling of ubiquitin conjugation to endoplasmic reticulum degradation) domain is an evolutionarily conserved, ~40 amino acid monoubiquitin-binding domain that mediates intramolecular monoubiquitylation. CUE domains are present in eukaryotic proteins that are involved in ubiquitination and protein trafficking pathways and may be required for ubiquitination of the proteins in which they are found. CUEDC2 (CUE domain-containing protein 2) was found through a yeast two-hybrid screening as a protein that interacts with the progesterone receptor (PR) and promotes progesterone-induced PR degradation by the ubiquitin-proteasome pathway. CUEDC2 also decreases the sumoylation of PR. CUEDC2 has also been found to interact with IKK-alpha and IKK-beta and decrease the activation of NF-κB by decreasing the activation of IKK.
Shih SC, Prag G, Francis SA, et al. A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. EMBO J. 2003; 22:1273-81.
Ponting CP. Proteins of the endoplasmic-reticulum-associated degradation pathway: domain detection and function prediction. Biochem. J. 2000; 351:527-35.
Prag G, Misra S, Jones EA, et al. Mechanism of ubiquitin recognition by the CUE domain of Vps9p. Cell 2003; 113:609-20.
Kang RS, Daniels CM, Francis SA, et al. Solution structure of a CUE-ubiquitin complex reveals a conserved mode of ubiquitin binding. Cell 2003; 113:621-30.
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