|Application ||WB, E|
|Other Accession||NP_861449, 189571669|
|Calculated MW||47558 Da|
|Application Notes||Spred2 antibody can be used for detection of Spred2 by Western blot at 1 - 2 µg/mL.|
|Target/Specificity||SPRED2; This Spred2 antibody is predicted to have no cross-reactivity to Spred1 or Spred3.|
|Reconstitution & Storage||Spred2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Spred2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase.|
|Cellular Location||Membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasm. Note=Especially found in the cytoplasm of the stratum spinosum cells called membrane-coated granules|
|Tissue Location||Expressed in liver, skin, small intestine, salivary gland and prostate.|
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Provided below are standard protocols that you may find useful for product applications.
Spred2 Antibody: Spred2 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1, Spred2 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. At least two isoforms of Spred2 are known to exist.
Wakioka T, Sasaki A, Kato R, et al. Spred is a Sprouty-related suppressor of Ras signalling. Nature2001; 412:647-51.
Casci T, Vinos J, and Freeman M. Sprouty, an intracellular inhibitor of Ras signaling. Cell1999; 96:655-65.
Minowada G, Jarvis LA, Chi CL, et al. Vertebrate Sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed. Development1999; 126:4465-75.
Lee SH, Schloss DJ, Jarvis L, et al. Inhibition of angiogenesis by a mouse sprouty protein. J. Biol. Chem.2001; 276:4128-33.
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