|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001035987, 399473|
|Calculated MW||42670 Da|
|Application Notes||Spred3 antibody can be used for detection of Spred3 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.|
|Target/Specificity||Spred3 antibody was raised against a 16 amino acid synthetic peptide near the center of the human Spred3.|
The immunogen is located within amino acids 140 - 190 of Spred3.
|Reconstitution & Storage||Spred3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Spred3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase.|
|Cellular Location||Membrane; Peripheral membrane protein|
|Tissue Location||Expression is restricted to liver.|
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Provided below are standard protocols that you may find useful for product applications.
Spred3 Antibody: Spred3 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1 and Spred2, Spred3 contains and EVH1 domain in its amino terminus and a Sprouty-related cysteine-rich (SPR) domain in its C-terminus, but does not possess a functional c-kit binding domain (KBD). The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Although Spred3 was initially reported to be expressed in brain, at least one report also indicates that Spred3 can be found in liver. At least two isoforms of Spred3 are known to exist.
Kato R, Nonami A, Taketomi T, et al. Molecular cloning of mammalian Spred-3 which suppresses tyrosine kinase-mediated Erk activation. Biochem. Biophys. Res. Commun.2003; 302:767-72.
Casci T, Vinos J, and Freeman M. Sprouty, an intracellular inhibitor of Ras signaling. Cell1999; 96:655-65.
Minowada G, Jarvis LA, Chi CL, et al. Vertebrate Sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed. Development1999; 126:4465-75.
Lee SH, Schloss DJ, Jarvis L, et al. Inhibition of angiogenesis by a mouse sprouty protein. J. Biol. Chem.2001; 276:4128-33.
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