|Application ||IHC-P, E|
|Other Accession||A7MBM2, 160380692|
|Calculated MW||152003 Da|
|Application Notes||DISP2 antibody can be used for detection of DISP2 by immunohistochemistry at 5 µg/mL.|
|Target/Specificity||DISP2; DISP2 antibody is human specific. At least two isoforms of Disp2 are known to exist. DISP2 antibody is predicted to not cross-react with Disp1 or Disp3.|
|Reconstitution & Storage||DISP2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||DISP2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Membrane; Multi-pass membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
DISP2 Antibody: DISP2 is the second of three known homologs of the D. melanogaster protein Dispatched. It is a multi-transmembrane protein containing two PTCH/DISP domains and is thought to be involved in the release of lipid-anchored Hedgehog from producing cells. Hedgehog is a major player in signaling pathways during embryogenesis, tissue regeneration, and carcinogenesis and the DISP proteins have been implicated in these pathways. Recently, it has been shown that DISP2 is translationally regulated by the microRNA miR-214 in zebrafish. Expression of this miRNA decreased DISP2 promoter activity in vitro and its overexpression in zebrafish resulted in a phenotype identical to that observed by DISP2 mutants.
Katoh Y and Katoh M. Identification and characterization of DISP3 gene in silico. Int. J. Oncol. 2005; 26:551-6.
Katoh Y and Katoh M. Hedgehog signaling pathway and gastric cancer. Can. Biol. & Ther. 2005; 4:1050-4.
Li N, Flynt AS, Kim HR, et al. Dispatched homolog 2 is targeted by miR-214 through a combination of three week microRNA recognition sites. Nuc. Acids Res. 2008; 36:4277-85.
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