|Application ||WB, IHC-P, E|
|Other Accession||O75323, 17380133|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||33743 Da|
|Application Notes||NIPSNAP2 antibody can be used for detection of NIPSNAP2 by Western blot at 0.5 and 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL.|
|Reconstitution & Storage||NIPSNAP2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||NIPSNAP2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Tissue Location||Widely expressed. Most abundant in heart and skeletal muscle|
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Provided below are standard protocols that you may find useful for product applications.
NIPSNAP2 Antibody: The NIPSNAP proteins comprise a family of evolutionarily well-conserved proteins with strong sequence similarity to the central portion of a protein encoded by C. elegans chromosome III between a 4-nitrophenylphosphatase (NIP) domain and non-neuronal SNAP25-like protein. NIPSNAP2, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR in approximately 40% of glioblastomas, the most common and malignant form of central nervous system tumors. It is widely expressed and most abundant in heart and skeletal muscle. NIPSNAP proteins have been suggested to be important in vesicular transport. NIPSNAP2 antibody is predicted to not cross-react with any other members of the NIPSNAP protein family.
Seroussi E, Pan HQ, Kedra D, et al. Characterization of the human NIPSNAP1 gene from 22q12: a member of a novel gene family. Gene1998; 212:13-20.
Wang X-Y, Smith DI, Liu W, et al. GBAS, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR. Genomics1998; 49:448-51.
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