|Application ||WB, IF, ICC, E|
|Other Accession||NP_612208, 19923881|
|Calculated MW||31085 Da|
|Application Notes||GNPDA2 antibody can be used for detection of GNPDA2 by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 4 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||GNPDA2; GNPDA2 antibody is predicted to not cross-react with GNPDA1.|
|Reconstitution & Storage||GNPDA2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||GNPDA2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
GNPDA2 Antibody: Glucosamine-6-phosphate deaminase (GNPDA) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. GNPDA2 is the second mammalian glucosamine-6-phosphate deaminase discovered, and is closer in structure and activity to the E. coli enzyme than GNPDA1. GNPDA1 possesses greater affinity for ammonium than either GNPDA2 or the E. coli enzyme suggesting that the forward reaction of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium is catalyzed at a slower rate than GNPDA2.
Wolosker H, Kline D, Bian Y, et al. Molecularly cloned mammalian glucosamine-6-phosphate deaminase localizes to transporting epithelium and lacks oscillin activity. FASEB J.1998; 12:91-9.
Zhang J, Zhang W, Zou D, et al. Cloning and functional characterization of GNPI, a novel human homolog of glucosamine-6-phosphate isomerase/oscillin. J. Cell Biochem.2003; 88:932-40.
Arreola R, Valderrama B, Morante ML, et al. Two mammalian glucosamine-6-phosphate deaminases: a structural and genetic study. FEBS Lett.2003; 551:63-70.
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