|Application ||WB, IF, ICC, E|
|Other Accession||NP_001096123, 54726|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 115, 123 kDa |
Observed: 110 kDa
|Application Notes||OTUD4 antibody can be used for detection of OTUD4 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.|
|Target/Specificity||OTUD4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human OTUD4.|
The immunogen is located within the last 50 amino acids of OTUD4.
|Reconstitution & Storage||OTUD4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||OTUD4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Deubiquitinating enzyme that specifically hydrolyzes 'Lys-48'-linked polyubiquitin.|
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Provided below are standard protocols that you may find useful for product applications.
OTUD4 Antibody: OTUD4, also known as HIV-1 induced protein HIN-1, is a member of the OTU (ovarian tumor) domain containing cysteine protease superfamily, in which the OUT domain generally confers deubiquitinase activity. At least three isoforms of OTUD4 are known to exist, and the smallest of these isoforms are only expressed in HIV-1-infected cells (provided by RefSeq).
Borodovsky A, Ovaa H, Kolli N, et al. Chemistry-based functional genomics reveals novel members of the deubiquitinating enzyme family. Chem. Biol. 2002; 10:1149-59.
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