|Application ||WB, E|
|Other Accession||AAH15030, 15929151|
|Calculated MW||78156 Da|
|Application Notes||ZBED1 antibody can be used for detection of ZBED1 by Western blot at 1 µg/mL.|
|Target/Specificity||ZBED1; This antibody is predicted to have no cross-reactivity to ZBED2 or ZBED3.|
|Reconstitution & Storage||ZBED1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||ZBED1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ALTE, DREF, KIAA0785, TRAMP|
|Function||Binds to 5'-TGTCG[CT]GA[CT]A-3' DNA elements found in the promoter regions of a number of genes related to cell proliferation. Binds to the histone H1 promoter and stimulates transcription. Was first identified as gene weakly similar to Ac transposable elements, but does not code for any transposase activity.|
|Cellular Location||Nucleus. Note=In granular structures|
|Tissue Location||Ubiquitously expressed at low levels. Expression is highest in skeletal muscle, heart, spleen and placenta|
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Provided below are standard protocols that you may find useful for product applications.
ZBED1 Antibody: ZBED1, also known as DREF, is the human homolog of the Drosophila protein DREF, a transcriptional regulatory factor required for expression of genes involved in DNA replication and cell proliferation. RNA interference (RNAi) experiments targeting ZBED1 RNA resulted in the inhibition of S phase entry and reduction of histone H1 mRNA in HeLa cells, suggesting that ZBED1 also plays a role in cell proliferation in human cells. At least 22 ribosomal proteins (RPs) possess ZBED1 binding sites in their promoters; anti-ZBED1 RNAi also resulted in decreased RP gene expression. Immunoprecipitation analyses have shown that ZBED1 self-associates in vivo via a carboxyl-terminal hATC domain, and this self-association is required for nuclear localization and DNA binding.
Ohshima N, Takahashi M, and Hirose F. Identification of a human homolog of the DREF transcription factor with a potential role in regulation of the histone H1 gene. J. Biol. Chem.2003; 278:22928-38.
Yamashita D, Sano Y, Adachi Y, et al. hDREF regulates cell proliferation and expression of ribosomal protein genes. Mol. Cell. Biol.2007; 27:2003-13.
Yamashita D, Komori H, Higuchi Y, et al. Human DNA replication-related element binding factor (hDREF) self-association via hATC domain is necessary for its nuclear accumulation and DNA binding. J. Biol. Chem.2007; 282:7563-75.
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