ZBED1 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | O96006 |
Other Accession | AAH15030, 15929151 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 78156 Da |
Application Notes | ZBED1 antibody can be used for detection of ZBED1 by Western blot at 1 µg/mL. |
Gene ID | 9189 |
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Target/Specificity | ZBED1; This antibody is predicted to have no cross-reactivity to ZBED2 or ZBED3. |
Reconstitution & Storage | ZBED1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | ZBED1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ZBED1 |
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Function | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase which sumoylates CHD3/Mi2-alpha, causing its release from DNA (PubMed:27068747). This results in suppression of CHD3/Mi2-alpha transcription repression, increased recruitment of RNA polymerase II to gene promoters and positive regulation of transcription including H1-5 and ribosomal proteins such as: RPS6, RPL10A, and RPL12 (PubMed:12663651, PubMed:17209048, PubMed:17220279, PubMed:27068747). The resulting increased transcriptional activity drives cell proliferation (PubMed:12663651, PubMed:17220279). Binds to 5'- TGTCG[CT]GA[CT]A-3' consensus sequences in gene promoters of ribosomal proteins (PubMed:12663651, PubMed:17209048, PubMed:17220279, PubMed:27068747). |
Cellular Location | Nucleus, PML body. Nucleus. Note=In granular structures |
Tissue Location | Ubiquitously expressed at low levels (PubMed:9887332). Expression is highest in skeletal muscle, heart, spleen and placenta (PubMed:9887332). |
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Provided below are standard protocols that you may find useful for product applications.
Background
ZBED1 Antibody: ZBED1, also known as DREF, is the human homolog of the Drosophila protein DREF, a transcriptional regulatory factor required for expression of genes involved in DNA replication and cell proliferation. RNA interference (RNAi) experiments targeting ZBED1 RNA resulted in the inhibition of S phase entry and reduction of histone H1 mRNA in HeLa cells, suggesting that ZBED1 also plays a role in cell proliferation in human cells. At least 22 ribosomal proteins (RPs) possess ZBED1 binding sites in their promoters; anti-ZBED1 RNAi also resulted in decreased RP gene expression. Immunoprecipitation analyses have shown that ZBED1 self-associates in vivo via a carboxyl-terminal hATC domain, and this self-association is required for nuclear localization and DNA binding.
References
Ohshima N, Takahashi M, and Hirose F. Identification of a human homolog of the DREF transcription factor with a potential role in regulation of the histone H1 gene. J. Biol. Chem.2003; 278:22928-38.
Yamashita D, Sano Y, Adachi Y, et al. hDREF regulates cell proliferation and expression of ribosomal protein genes. Mol. Cell. Biol.2007; 27:2003-13.
Yamashita D, Komori H, Higuchi Y, et al. Human DNA replication-related element binding factor (hDREF) self-association via hATC domain is necessary for its nuclear accumulation and DNA binding. J. Biol. Chem.2007; 282:7563-75.
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