|Application ||WB, ICC, E|
|Other Accession||NP_115743, 14150185|
|Calculated MW||25132 Da|
|Application Notes||ZBED3 antibody can be used for detection of ZBED3 by Western blot at 1 µg/mL. Despite its predicted molecular weight, ZBED3 often migrates at a higher position in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 20 µg/mL.|
|Target/Specificity||ZBED3; This antibody is predicted to have no cross-reactivity to ZBED1 or ZBED2.|
|Reconstitution & Storage||ZBED3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||ZBED3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a positive regulator in the activation of the canonical Wnt/beta-catenin signaling pathway by stabilizing cytoplasmic beta-catenin. Involved in transcription activation of Wnt target gene expression (By similarity).|
|Cellular Location||Cytoplasm. Membrane. Note=Colocalizes with AXIN1 in the cytoplasm|
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Provided below are standard protocols that you may find useful for product applications.
ZBED3 Antibody: Axin is a key modulator of the Wnt/beta-catenin pathway and acts as a scaffold protein in phosphorylating and degrading cytoplasmic beta-catenin. ZBED3, a member of the Zinc finger BED-type domain containing superfamily, has been recently identified as an Axin-interacting protein and has been suggested to modulate the Wnt/beta-catenin signaling pathway. RNA interference (RNAi) experiments targeting ZBED3 RNA attenuated Wnt-induced beta-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and Wnt gene expression.
Chen T, Li M, Ding Y, et al. Identification of Zinc-finger BED domain containing 3 (Zbed3) as a novel axin-interacting protein that activates Wnt/b-catenin signaling. J. Biol. Chem.2009; 284:6683-9.
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