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ZBED3 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC, E |
|---|---|
| Primary Accession | Q96IU2 |
| Other Accession | NP_115743, 14150185 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 25132 Da |
| Application Notes | ZBED3 antibody can be used for detection of ZBED3 by Western blot at 1 µg/mL. Despite its predicted molecular weight, ZBED3 often migrates at a higher position in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 20 µg/mL. |
| Gene ID | 84327 |
|---|---|
| Target/Specificity | ZBED3; This antibody is predicted to have no cross-reactivity to ZBED1 or ZBED2. |
| Reconstitution & Storage | ZBED3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | ZBED3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ZBED3 |
|---|---|
| Function | Acts as a positive regulator in the activation of the canonical Wnt/beta-catenin signaling pathway by stabilizing cytoplasmic beta-catenin (By similarity). Involved in transcription activation of Wnt target gene expression (By similarity). Plays a role in symmetric division of blastomeres in the early stages of embryogenesis via regulation of mitotic spindle central positioning and organization of the F-actin filament network (By similarity). Plays a role in regulating the distribution of cellular organelles, via modulation of cytoskeletal dynamics and cytoplasmic lattice formation (By similarity). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q9D0L1}. Membrane {ECO:0000250|UniProtKB:Q9D0L1}. Secreted |
| Tissue Location | Secreted in blood plasma, and expressed in skeletal muscle and adipose tissue (at protein level) |
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Provided below are standard protocols that you may find useful for product applications.
Background
ZBED3 Antibody: Axin is a key modulator of the Wnt/beta-catenin pathway and acts as a scaffold protein in phosphorylating and degrading cytoplasmic beta-catenin. ZBED3, a member of the Zinc finger BED-type domain containing superfamily, has been recently identified as an Axin-interacting protein and has been suggested to modulate the Wnt/beta-catenin signaling pathway. RNA interference (RNAi) experiments targeting ZBED3 RNA attenuated Wnt-induced beta-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and Wnt gene expression.
References
Chen T, Li M, Ding Y, et al. Identification of Zinc-finger BED domain containing 3 (Zbed3) as a novel axin-interacting protein that activates Wnt/b-catenin signaling. J. Biol. Chem.2009; 284:6683-9.
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