|Application ||WB, IHC-P, IF, E|
|Other Accession||P02768, 213|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||69367 Da|
|Application Notes||Albumin antibody can be used for detection of Albumin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.|
|Target/Specificity||Albumin antibody was raised against a 13 amino acid synthetic peptide near the center of human Albumin.|
The immunogen is located within amino acids 340 - 390 of Albumin.
|Reconstitution & Storage||Albumin antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Albumin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.|
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Provided below are standard protocols that you may find useful for product applications.
Albumin Antibody: Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. It functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The resulting product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. At least two isoforms of Albumin are known to exist.
Rothschild MA, Oratz M, and Schreiber SS. Serum Albumin. Hepatology 1988; 8:385-401.
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