|Application ||WB, IHC-P, IF, E|
|Other Accession||AAH29886, 4950|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 57 kDa |
Observed: 57 kDa
|Application Notes||OCLN antibody can be used for detection of OCLN by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.|
|Target/Specificity||OCLN antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human OCLN.|
The immunogen is located within the last 50 amino acids of OCLN.
|Reconstitution & Storage||OCLN antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||OCLN Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.|
|Cellular Location||Cell membrane; Multi- pass membrane protein. Cell junction, tight junction|
|Tissue Location||Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis|
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Provided below are standard protocols that you may find useful for product applications.
OCLN Antibody: Tight junctions act as a semi-permeable barrier to the transport of ions, solutes, and water and are considered to function as a fence that divides apical and basolateral domains of plasma membranes. Tight junctions coordinate a variety of signaling and trafficking molecules regulating cell differentiation, proliferation, and polarity and contain a number of junctional proteins including Occludin, Claudins, junctional adhesion molecules (JAMs), as well as multiple scaffold proteins. Occludin, the first identified component of tight junction strands, is thought function as a signal transmitter in multiple signaling pathways and can associate with multiple kinases and phosphatases such as PI3-kinase and protein phosphatases 1 and 2A. At least two isoforms of OCLN are known to exist.
Tsukita S, Furuse M, and Itoh M. Multifunctional strands in tight junctions. Nat. Rev. Mol. Cell Biol. 2001; 2:285-93.
Chiba H, Osanai M, Murata M, et al. Transmembrane proteins of tight junctions. Biochim. Biophys. Acta 2008; 1778:588-600.
Furuse M, Hirase T, Itoh M, et al. Occludin: a novel integral membrane protein localizing at tight junctions. J. Cell Biol.1993; 1777-88.
Matter K, Aijaz S, Tsapara A, et al. Mammalian tight junctions in the regulation of epithelial differentiation and proliferation. Curr. Opin. Cell Biol. 2005; 17:453-8.
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