|Application ||WB, IF, ICC, E|
|Other Accession||NP_085127, 226442729|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||69775 Da|
|Application Notes||KLHL15 antibody can be used for detection of KLHL15 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||KLHL15 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||KLHL15 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Substrate-specific adapter for CUL3 E3 ubiquitin-protein ligase complex (PubMed:14528312). Acts as an adapter for CUL3 to target the serine/threonine-protein phosphatase 2A (PP2A) subunit PPP2R5B for ubiquitination and subsequent proteasomal degradation, thus promoting exchange with other regulatory subunits (PubMed:23135275). Acts as an adapter for CUL3 to target the DNA- end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, plays a key role in DNA damage response, favoring DNA double-strand repair through error-prone non- homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (PubMed:27561354).|
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Provided below are standard protocols that you may find useful for product applications.
KLHL15 Antibody: KLHL15 is a member of a growing superfamily of kelch repeat-containing proteins. The kelch motif was discovered in the sequence of the Drosophila kelch ORF1 protein and forms a conserved beta-propeller tertiary structure. These kelch-like proteins have been implicated in embryogenesis and carcinogenesis through cytoskeleton organization. KLHL15 mRNA is widely expressed in many tissues and is thought to be involved in protein ubiquination and cytoskeleton organization.
Yoshida K. Identification and characterization of a novel kelch-like gene KLHL15 in silico. Oncol. Rep.2005; 13:1133-7.
Robinson DN and Cooley L. Drosophila kelch is an oligomeric ring canal actin organizer. J. Cell Biol.1997; 138:799-810.
Adams J, Kelso R, and Cooley L. The kelch repeat superfamily of proteins: propellers of cell function. Trends Cell Biol.2000; 10:17-24.
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