|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_079097, 13376216|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||26561 Da|
|Application Notes||CCDC134 antibody can be used for detection of CCDC134 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||CCDC134 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||CCDC134 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||In extracellular secreted form, promotes proliferation and activation of CD8(+) T cells, suggesting a cytokine-like function (PubMed:25125657). Enhances cytotoxic anti-tumor activity of CD8(+) T cells (PubMed:25125657). May inhibit ERK and JNK signaling activity (PubMed:18087676, PubMed:23070808). May suppress cell migration and invasion activity, via its effects on ERK and JNK signaling (PubMed:23070808).|
|Cellular Location||Nucleus. Cytoplasm. Secreted. Endoplasmic reticulum. Note=Accumulates in the nucleus in response to UV irradiation (PubMed:22644376)|
|Tissue Location||Expressed in cervical gland, cervical squamous epithelium, endometrium, stomach, kidney distal convoluted tubule, spermatogenic cells in testis, mammary gland, liver and striated muscle (at protein level) (PubMed:18087676, PubMed:23070808). Also detected in placenta (PubMed:18087676). Highest expression in testis relative to other tissues (PubMed:18087676). Detected in T cells and dendritic cells; highly expressed in activated CD8(+) T cells, and also expressed at lower levels in CD4(+) T cells (PubMed:25125657).|
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Provided below are standard protocols that you may find useful for product applications.
CCDC134 Antibody: The coiled-coil domain is a common protein motif that is often involved in protein oligomerization and is found in proteins such as transcription factors and intermediate filaments. One such protein is CCDC134, a recently identified secretory protein that has been found to inhibit the transcriptional activity of the Elk1 protein. Overexpression CCDC134 also inhibited the phosphorylation of Erk and JNK/SAPK but not p38 MAPK, while specific siRNA against CCDC134 activated Elk1 transcriptional activity and the phosphorylation of Erk and JNK/SAPK, suggesting a potential inhibiting role of CCDC134 in MAPK-mediated Elk1 transcription. CCDC134 is widely expressing in normal adult tissues, tumors, and cell lines.
Steinmetz MO, Jelesarov I, Matousek WM, et al. Molecular basis of coiled-coil formation. Proc. Natl. Acad. Sci. USA2007; 104:7062-7.
Huang J, Shi T, Ma T, et al. CCDC134, a novel secretory protein, inhibits activation of ERK and JNK, but not p38 MAPK. Cell. Mol. Life Sci.2008; 65:338-49.
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