|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_524558, 17738287|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||25728 Da|
|Application Notes||SCAMP4 antibody can be used for detection of SCAMP4 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||SCAMP4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SCAMP4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Probably involved in membrane protein trafficking.|
|Cellular Location||Membrane; Multi-pass membrane protein.|
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Provided below are standard protocols that you may find useful for product applications.
SCAMP4 Antibody: Secretory carrier membrane proteins (SCAMPs) are integral membrane proteins seen in secretory and endocytic carriers that are thought to play roles in membrane trafficking. One member of this family, SCAMP4, lacks the putative calcium binding and leucine zipper structures as well as the protein-protein interacting NPF domains common to most SCAMP family members. In rats, SCAMP4 mRNA is repressed by progesterone in brain regions associated with female sexual behavior. At least three isoforms of SCAMP4 are known to exist. This SCAMP4 antibody is predicted to not cross-react with any other member of the secretory carrier membrane protein family.
Hubbard C, Singleton D, Rauch M, et al. The secretory carrier membrane protein family: structure and membrane topology. Mol. Biol. Cell2000; 11:2933-47.
Krebs CJ and Pfaff DW. Expression of the SCAMP-4 gene, a new member of the secretory carrier membrane protein family, is repressed by progesterone in brain regions associated with female sexual behavior. Brain Res. Mol. Brain Res.2001; 88:144-54.
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