|Application ||WB, IF, ICC, E|
|Other Accession||NP_062558, 10257429|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||50288 Da|
|Application Notes||IRGC antibody can be used for detection of IRGC by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||IRGC; Two isoforms of IRGC are known to exist; this antibody will recognize both isoforms.|
|Reconstitution & Storage||IRGC antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||IRGC Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
IRGC Antibody: Immunity-related GTPases (IRG) (also known as p47 GTPases) are a family of GTPase proteins found in vertebrates, which play critical roles in mediating innate resistance to intracellular pathogens. IRG genes have been found in a number of mammals and lower species including mice, rats, zebrafish and humans. Most of the mouse genes contain interferon-stimulated response elements which mediate transcriptional activation by IFNs. In humans, only two IRG genes have been found: human IRGC encodes a full-length IRG protein that, like the mouse homologue, is constitutively expressed in testis, while human IRGM encodes a considerably truncated protein that is constitutively expressed in cultured cells including some macrophage cell lines. As the two human genes IRGC and IRGM are not subject to IFN control, it has been suggested that the host resistance mechanism supported by IRG proteins in the mouse is lacking in humans.
Taylor GA. IRG proteins: key mediators of interferon-regulated host resistance to intracellular pathogens. Cell Microbiol.2007; 9:1099-107.
Yamada K, Akimoto H, Ogawa Y, et al. Upregulation of immunity-related GTPase (IRG) proteins by TNF-alpha in murine astrocytes. Biochem. Biophys. Res. Commun.2009; 382:434-9.
Singh SB, et al. Human IRGM induces autophagy to eliminate intracellular mycobacteria. Science2006; 313:1438-41.
Bekpen C, Hunn JP, Rohde C, et al. The interferon-inducible p47 (IRG) GTPases in vertebrates: loss of the cell autonomous resistance mechanism in the human lineage. Genome Biol.2005; 6:R92.
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