|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_116165, 118600981|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||284525 Da|
|Application Notes||JMJD1C antibody can be used for detection of JMJD1C by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||JMJD1C; This antibody will not cross-react with JMJD1A or JMJD1B.|
|Reconstitution & Storage||JMJD1C antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||JMJD1C Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||JHDM2C, KIAA1380, TRIP8|
|Function||Probable histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May be involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
JMJD1C Antibody: The jumonji domain containing 1C protein (JMJD1C) was initially discovered in silico, and later suggested to be a candidate gene for autism. Like the related proteins JMJD1A and JMJD1B, JMJD1C is a histone H3K9 demethylase implicated in the nuclear hormone receptor-based transcriptional regulation. JMJD1C mRNA is highly expressed in undifferentiated embryonic stem (ES) cells as well as pancreatic islet, diffuse-type gastric cancer, and other tissues and tumors. The JMJD1C gene promoter contain bHLH-, AP-1-, and POU5F1-binding sites, and as preferential expression of POU5F1 has been reported in ES cells, pancreatic islet, and diffuse-type gastric cancer, it has been suggested that POU5F1-mediated expression of JMJD1C reactivates previously silenced genes in ES cells and diffuse-type gastric cancer. At least three isoforms of JMJD1C are known to exist.
Katoh M and Katoh M. Identification of TRIP8 gene in silico. Int. J. Mol. Med.2003; 12:817-21.
Castermans D, Vermeesch JR, Fryns JP, et al. Identification and characterization of the TRIP8 and REEP3 genes on chromosome 10q21.3 as novel candidate genes for autism. Eur. J. Hum. Genet.2007; 15:422-31.
Katoh M and Katoh M. Comparative integromics on JMJD1C gene encoding histone demethylase: Conserved POU5F1 binding site elucidating mechanism of JMJD1C expression in undifferentiated ES cells and diffuse-type gastric cancer. Int. J. Oncology2007; 31:219-23.
Katoh Y and Katoh M. Conserved POU-binding site linked to SP1-binding site within FZD5 promoter: transcriptional mechanism of FZD5 in undifferentiated human ES cells, fetal liver/spleen, adult colon, pancreatic islet, and diffuse-type gastric cancer. Int. J. Oncol.2007; 30:751-5.
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