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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RIPK1 Antibody   

RIPK1 Antibody

  • WB - RIPK1 Antibody ASC10976
    Western blot analysis of RIPK1 in rat kidney tissue lysate with RIPK1 antibody at 1 µg/mL.
  • IHC - RIPK1 Antibody ASC10976
    Immunohistochemistry of RIPK1 in mouse kidney tissue with RIPK1 antibody at 2.5 µg/mL.
  • IF - RIPK1 Antibody ASC10976
    Immunofluorescence of RIPK1 in Mouse Kidney cells with RIPK1 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13546
Other Accession NP_003795, 57242761
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 75931 Da
Application Notes RIPK1 antibody can be used for detection of RIPK1 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 8737
Target/Specificity RIPK1;
Reconstitution & Storage RIPK1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsRIPK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RIPK1
Synonyms RIP, RIP1
Function Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.
Cellular Location Cytoplasm. Cell membrane.
Research Areas
Citations (0)

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RIPK1 Antibody: RIPK1 (Receptor Interacting Protein) is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell's decision to live or die. RIPK1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIPK1 is important for signallling to NFκ-B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIPK1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF-κB activation. RIPK1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.


Stanger BZ, Leder P, Lee TH, et al. RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death. Cell1995; 81:513-23.
Hsu H, Huang J, Shu HB, et al. TNF-dependent recruitment of the protein kinase RIP to the TNF receptor-1 signaling complex. Immunity1996; 4:387-96.
Meylan E, Burns K, Hofmann K, et al. RIP1 is an essential mediator of Toll-like receptor 3-induced NF-kappa B activation. Nat. Immunol.2004; 5:503-7.
Festjens N, Vanden Bergh T, Cornelis S, et al. RIP1, a kinase on the crossroads of a cell's decision to live or die. Cell Death Differ.2007;14:400-10.

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$ 310.00
Cat# ASC10976
(40 western blots)
Availability: 5-7days
Bulk Size
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