|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_055546, 7662176|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||71791 Da|
|Application Notes||Prosapip1 antibody can be used for detection of Prosapip1 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||Prosapip1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||Prosapip1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cytoplasm, cytoskeleton. Note=Detected at synapses, postsynaptic density, synaptic spines and dendrites|
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Provided below are standard protocols that you may find useful for product applications.
Prosapip1 Antibody: Prosapip1 is a binding partner of the postsynaptic density (PSD) protein ProSAP2/Shank3, a major scaffolding protein in excitatory brain synapses. Prosapip1 is thought to bind ProSAP2/Shank3 through ProSAP2/Shank3's PDZ domain and link it in turn to the Spine-associated Rap-Gap (SPAR). Prosapip1 shares significant homology with another PSD protein, PSD-Zip70, with both coding for a Fez1 domain that can be found in a total of four related protein, forming a novel family of scaffolding molecules termed ‘Fezzins'. Recent studies show that Prosapip1 can be found in endocrine cells in which it is localized primarily in the hormone-storing compartment of these cells, suggesting that Prosapip1 may have other functions in non-neuronal cells.
Wendholt D, Spilker C, Schmitt A, et al. ProSAP-interacting protein 1 (ProSAPiP1), a novel protein of the postsynaptic density that links the spine-associated Rap-Gap (SPAR) to the scaffolding protein proSAP2/Shank3. J. Biol. Chem.2006; 281:13805-16.
Redecker P, Bockmann J, and Bockers TM. Secretory granules of hypophyseal and pancreatic endocrine cells contain proteins of the neuronal postsynaptic density. Cell Tissue Res.2007; 328:49-55.
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