|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_055888, 71143119|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||194610 Da|
|Application Notes||SIPA1L3 antibody can be used for detection of SIPA1L3 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||SIPA1L3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SIPA1L3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a critical role in epithelial cell morphogenesis, polarity, adhesion and cytoskeletal organization in the lens (PubMed:26231217).|
|Cellular Location||Apical cell membrane. Note=Detected in tricellular junctions. Colocalizes with apical F-actin|
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Provided below are standard protocols that you may find useful for product applications.
SIPA1L3 Antibody: Signal-induced proliferation associated-like protein 2 (SIPA1L3) is a member of the SIPA1 family of RapGAPs. SIPA1L3 was cloned as a novel molecular component of tight junctions and adherens junctions, suggesting that may function to balance Rap1 by counteracting RapGEFs. Recent studies of SIPA indicate that its deregulation can cause myeloproliferative stem cell disorders in mice and increased metastases in human cancers. Other studies suggest SIPA1L1 may play important roles in embryo development and control of cell proliferation. Based on the amount of homology between SIPA family members, it is possible that SIPA1L3 plays other roles development and cell proliferation.
Minato N and Hattori M. SPA-1 (Sipa1) and Rap signaling in leukemia and cancer metastasis. Cancer Sci.2009; 100:17-23.
Matsuda M, Kobayashi Y, Masuda S, et al. Identification of adherences junction-associated GTPase activating proteins by the fluorescence localization-based expression cloning. Exp. Cell Res.2008; 314:939-49.
Ishida D, Kometani K, Yang H, et al. Myeloproliferative stem cell disorders by deregulated Rap1 activation in SPA-1-deficient mice. Cancer Cell2003; 4:55-65.
Park YG, Zhao X, Lesueur F, et al. Sipa1 is a candidate for underlying the metastasis efficiency modifier locus, Mtes. Nat. Genet.2005; 37:1055-62.
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