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PICALM Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, E |
|---|---|
| Primary Accession | Q13492 |
| Other Accession | NP_009097, 56788366 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 70755 Da |
| Application Notes | PICALM antibody can be used for detection of PICALM by Western blot at 1 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 8301 |
|---|---|
| Target/Specificity | PICALM; |
| Reconstitution & Storage | PICALM antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | PICALM Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PICALM |
|---|---|
| Synonyms | CALM |
| Function | Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly (PubMed:10436022, PubMed:16262731, PubMed:27574975). Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature (PubMed:25898166). In addition to binding to clathrin, mediates the endocytosis of small R- SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8 (PubMed:22118466, PubMed:21808019, PubMed:23741335). In turn, PICALM- dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors (PubMed:25241929). Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP- CTF) (PubMed:25241929, PubMed:24067654). |
| Cellular Location | Cell membrane. Membrane, clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle, clathrin- coated vesicle. Nucleus. Note=Colocalized with clathrin in the Golgi area (PubMed:10436022). Interaction with PIMREG may target PICALM to the nucleus in some cells (PubMed:16491119) |
| Tissue Location | Expressed in all tissues examined. |
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Provided below are standard protocols that you may find useful for product applications.
Background
PICALM Antibody: PICALM, also known as the clathrin assembly lymphoid myeloid leukemia (CALM) protein, co-localizes with clathrin at clathrin-coated areas of the plasma membrane and in the Golgi area, and is thought to be an important component of the coated pit internalization machinery. Fusion proteins involving PICALM have been implicated in acute myeloid and T-cell acute lymphoblastic leukemia. PICALM is also expressed in neurons and is present at the synapse where it facilitates the endocytosis of the synaptic vesicle protein VAMP2. Recent studies have identified variants of PICALM associated with Alzheimer's disease and have suggested this protein may play a role in the endocytosis and recycling of the amyloid protein precursor (APP).
References
Tebar F, Bohlander SK, and Sorkin A. Clathrin assembly lymphoid myeloid leukemia (CALM) protein: localization in endocytic-coated pits, interactions with clathrin, and the impact of overexpression on clathrin mediated traffic. Mol. Biol. Cell1999; 10:2687-702.
Caudell D and Aplan PD. The role of CALM-AF10 gene fusion in acute leukemia. Leukemia2008; 22:678-85.
Harel A, Wu F, Mattson MP, et al. Evidence for CALM in directing VAMP2 trafficking. Traffic2008; 9:417-29.
Harold D, Abraham R, Hollingworth P, et al. Genome-wide association study identifies variants at CLU and PICALM associated with Alzheimer’s disease. Nat. Genet.2009; 41:1088-93.
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