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NANOG Antibody

  • WB - NANOG Antibody ASC11057
    Western blot analysis of NANOG in human spleen tissue lysate with NANOG antibody at (A) 1 and (B) 2 µg/mL.
  • IHC - NANOG Antibody ASC11057
    Immunohistochemistry of NANOG in human spleen tissue with NANOG antibody at 5 µg/mL.
  • IF - NANOG Antibody ASC11057
    Immunofluorescence of NANOG in Human Spleen cells with NANOG antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H9S0
Other Accession EAW88651, 119609057
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 34620 Da
Application Notes NANOG antibody can be used for detection of NANOG by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 79923
Target/Specificity NANOG;
Reconstitution & Storage NANOG antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsNANOG Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'- [CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108, ECO:0000269|PubMed:15983365}
Tissue Location Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
Research Areas
Citations (0)

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NANOG Antibody: Expression of NANOG is required for the maintenance of pluripotency in epiblast and embryonic stem (ES) cells as well as for the ability to maintain ES self-renewal independently of LIF/Stat3. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.


Chambers I, Colby D, Robertson M, et al. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell2003; 113:643-55.
Mitsui K, Tokuzowa Y, Itoh H, et al. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell2003; 113:631-42.
Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells2003; 5:79-88.
Scholer HR, Ruppert S, Suzuki N, et al. New type of POU domain in germ line-specific protein Oct-2. Nature1990; 344:435-9.

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$ 310.00
Cat# ASC11057
(40 western blots)
Availability: 5-7days
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