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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   BFAR Antibody   

BFAR Antibody

     
  • WB - BFAR Antibody ASC11060
    Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 µg/mL.
  • IHC - BFAR Antibody ASC11060
    Immunohistochemistry of BFAR in mouse kidney tissue with BFAR antibody at 5 µg/mL.
  • IF - BFAR Antibody ASC11060
    Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 µg/mL.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q9NZS9
Other Accession NP_057645, 7706091
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application Notes BFAR antibody can be used for detection of BFAR by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 51283
Target/Specificity BFAR;
Reconstitution & Storage BFAR antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsBFAR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BFAR
Synonyms BAR, RNF47
Function Apoptosis regulator. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors.
Cellular Location Endoplasmic reticulum membrane; Multi- pass membrane protein
Tissue Location Expressed highly in brain, moderately in small intestine, weakly in testes and only faintly in liver and skeletal muscle. Not expressed in heart, kidney, lung and spleen EMBL; AF173003; AAF59975.1; -; mRNA EMBL; AK291114; BAF83803.1; -; mRNA EMBL; AK300778; BAG62442.1; -; mRNA EMBL; AC009167; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; CH471112; EAW85105.1; -; Genomic_DNA EMBL; CH471112; EAW85108.1; -; Genomic_DNA EMBL; BC003054; AAH03054.1; -; mRNA CCDS; CCDS10554.1; -. [Q9NZS9-1] RefSeq; NP_057645.1; NM_016561.2. [Q9NZS9-1] RefSeq; XP_005255407.1; XM_005255350.2. [Q9NZS9-2] UniGene; Hs.435556; - ProteinModelPortal; Q9NZS9; - SMR; Q9NZS9; - BioGrid; 119435; 20 CORUM; Q9NZS9; - IntAct; Q9NZS9; 10 STRING; 9606.ENSP00000261658; - iPTMnet; Q9NZS9; - PhosphoSitePlus; Q9NZS9; - BioMuta; BFAR; - DMDM; 74753089; - EPD; Q9NZS9; - MaxQB; Q9NZS9; - PaxDb; Q9NZS9; - PeptideAtlas; Q9NZS9; - PRIDE; Q9NZS9; - ProteomicsDB; 83505; - DNASU; 51283; - Ensembl; ENST00000261658; ENSP00000261658; ENSG00000103429. [Q9NZS9-1] Ensembl; ENST00000619034; ENSP00000478190; ENSG00000275618. [Q9NZS9-1] GeneID; 51283; - KEGG; hsa:51283; - UCSC; uc002dco.4; human. [Q9NZS9-1] CTD; 51283; - DisGeNET; 51283; - EuPathDB; HostDB:ENSG00000103429.10; - GeneCards; BFAR; - HGNC; HGNC:17613; BFAR neXtProt; NX_Q9NZS9; - OpenTargets; ENSG00000103429; - PharmGKB; PA38460; - eggNOG; KOG4159; Eukaryota eggNOG; COG2802; LUCA GeneTree; ENSGT00390000005386; - HOGENOM; HOG000285958; - HOVERGEN; HBG056789; - InParanoid; Q9NZS9; - KO; K15684; - OMA; WASLYRD; - OrthoDB; EOG091G06BG; - PhylomeDB; Q9NZS9; - TreeFam; TF332303; - SIGNOR; Q9NZS9; - ChiTaRS; BFAR; human GenomeRNAi; 51283; - PRO; PR:Q9NZS9; - Proteomes; UP000005640; Chromosome 16 Bgee; ENSG00000103429; - CleanEx; HS_BFAR; - ExpressionAtlas; Q9NZS9; baseline and differential Genevisible; Q9NZS9; HS GO; GO:0005783; C:endoplasmic reticulum; IDA:ParkinsonsUK-UCL GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; NAS:ParkinsonsUK-UCL GO; GO:0005887; C:integral component of plasma membrane; NAS:ParkinsonsUK-UCL GO; GO:0016020; C:membrane; IDA:MGI GO; GO:0089720; F:caspase binding; IDA:ParkinsonsUK-UCL GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW GO; GO:0030674; F:protein binding, bridging; IDA:ParkinsonsUK-UCL GO; GO:0061630; F:ubiquitin protein ligase activity; IDA:ParkinsonsUK-UCL GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW GO; GO:0043066; P:negative regulation of apoptotic process; IDA:MGI GO; GO:1903895; P:negative regulation of IRE1-mediated unfolded protein response; IMP:ParkinsonsUK-UCL GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IDA:ParkinsonsUK-UCL GO; GO:0051865; P:protein autoubiquitination; IDA:ParkinsonsUK-UCL GO; GO:0070936; P:protein K48-linked ubiquitination; IDA:ParkinsonsUK-UCL GO; GO:0070534; P:protein K63-linked ubiquitination; IDA:ParkinsonsUK-UCL GO; GO:0000209; P:protein polyubiquitination; IDA:ParkinsonsUK-UCL GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IDA:ParkinsonsUK-UCL Gene3D; 3.30.40.10; -; 1 InterPro; IPR001660; SAM InterPro; IPR013761; SAM/pointed_sf InterPro; IPR001841; Znf_RING InterPro; IPR013083; Znf_RING/FYVE/PHD InterPro; IPR017907; Znf_RING_CS Pfam; PF00536; SAM_1; 1 SMART; SM00184; RING; 1 SMART; SM00454; SAM; 1 SUPFAM; SSF47769; SSF47769; 1 PROSITE; PS50105; SAM_DOMAIN; 1 PROSITE; PS00518; ZF_RING_1; 1 PROSITE; PS50089; ZF_RING_2; 1 1: Evidence at protein level; Alternative splicing; Apoptosis; Complete proteome; Endoplasmic reticulum; Glycoprotein; Membrane; Metal-binding; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix; Zinc; Zinc-finger CHAIN 1 450 Bifunctional apoptosis regulator /FTId=PRO_0000055822 TOPO_DOM 1 140 Cytoplasmic. TRANSMEM 141 161 Helical. TOPO_DOM 162 331 Lumenal. TRANSMEM 332 352 Helical. TOPO_DOM 353 360 Cytoplasmic. TRANSMEM 361 381 Helical. TOPO_DOM 382 404 Lumenal. TRANSMEM 405 425 Helical. TOPO_DOM 426 450 Cytoplasmic. DOMAIN 182 249 SAM. {ECO:0000255|PROSITE- ProRule:PRU00184} ZN_FING 34 74 RING-type. {ECO:0000255|PROSITE- ProRule:PRU00175} CARBOHYD 232 232 N-linked (GlcNAc...) asparagine VAR_SEQ 1 28 MEEPQKSYVNTMDLERDEPLKSTGPQIS -> MVGIFKENR MSRMQRKMGRFPQSQYSPQ (in isoform 2) /FTId=VSP_055879 VAR_SEQ 29 156 Missing (in isoform 2). /FTId=VSP_055880 VARIANT 140 140 M -> R (in dbSNP:rs11546303) /FTId=VAR_052075 VARIANT 245 245 R -> H (in dbSNP:rs35377618) /FTId=VAR_052076 SEQUENCE 450 AA; 52738 MW; A662454FC2806CE6 CRC64; MEEPQKSYVN TMDLERDEPL KSTGPQISVS EFSCHCCYDI LVNPTTLNCG HSFCRHCLAL WWASSKKTEC PECREKWEGF PKVSILLRDA IEKLFPDAIR LRFEDIQQNN DIVQSLAAFQ KYGNDQIPLA PNTGRANQQM GGGFFSGVLT ALTGVAVVLL VYHWSSRESE HDLLVHKAVA KWTAEEVVLW LEQLGPWASL YRERFLSERV NGRLLLTLTE EEFSKTPYTI ENSSHRRAIL MELERVKALG VKPPQNLWEY KAVNPGRSLF LLYALKSSPR LSLLYLYLFD YTDTFLPFIH TICPLQEDSS GEDIVTKLLD LKEPTWKQWR EFLVKYSFLP YQLIAEFAWD WLEVHYWTSR FLIINAMLLS VLELFSFWRI WSRSELKTVP QRMWSHFWKV STQGLFVAMF WPLIPQFVCN CLFYWALYFN PIINIDLVVK ELRRLETQVL
Research Areas
Citations (0)

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Background

BFAR Antibody: The bifunctional apoptosis inhibitor (BFAR) is scaffold protein that integrates signaling components of the cells apoptosis-regulatory machinery. BFAR is a multidomain protein capable of inhibiting apoptosis induced by TNF-family death receptors ('extrinsic pathway') as well as mitochondria-dependent apoptosis ('intrinsic pathway'). Interaction of BFAR with Bcl-2 or Bcl-XL via a SAM domain may contribute to the anti-apoptotic properties of BFAR. In addition, BFAR contains a DED-like domain that is capable of suppressing apoptosis mediated at the receptor level. BFAR is also thought to be involved in the regulation of neuronal survival.

References

Zhang H, Xu Q, Krajewski S, et al. BAR: an apoptosis regulator at the intersection of caspases and Bcl-2 family proteins. Proc. Natl. Acad. Sci. USA2000; 97:2597-602.
Roth W, Kermer P, Krajewska M, et al. Bifunctional apoptosis inhibitor (BAR) protects neurons from diverse cell death pathways. Cell Death Differ. 2003; 10: 1178-87.

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Cat# ASC11060
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