|Application ||WB, IHC-P, IF, E|
|Other Accession||EAW75575, 119595981|
|Calculated MW||61709 Da|
|Application Notes||BCAS1 antibody can be used for detection of BCAS1 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||BCAS1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||BCAS1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Tissue Location||Expressed in brain and prostate, and at lower levels in testis, intestine and colon. Overexpressed in most breast cancer cell lines and down-regulated in some colorectal tumors.|
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Provided below are standard protocols that you may find useful for product applications.
BCAS1 Antibody: BCAS1 was identified through positional cloning and was found to be overexpressed in most but not all breast cancer cells lines. Little is known about this protein; BCAS1 is cytoplasmically localized and is thought to form homodimers through coiled-coil structures. High levels of BCAS1 mRNA are seen in brain and prostate tissues, with lower amounts observed in colon, intestine and testis. Cells engineered to overexpress BCAS1 did not lose anchorage-dependent growth or increase their rate of growth, suggesting that BCAS1 is not a prototypical oncogene. The BCAS1 gene was also found to be amplified in other carcinomas such as pancreatic carcinoma, suggesting that BCAS1 may play in important role in the control of cell proliferation.
Collins C, Rommens JM, Kowbel D, et al. Positional cloning of ZNF217 and NABC1: genes amplified at 20q13.2 and overexpressed in breast carcinoma. Proc. Natl. Acad. Sci. USA1998; 95:8703-8.
Beardsley DI, Kowbel D, Lataxes TA, et al. Characterization of the novel amplified in breast cancer-1 (NABC1) gene product. Exp. Cell Res.2003; 290:402-13.
Loukopoulos P, Shibata T, Katoh H, et al. Genome-wide assay-based comparative genomic hybridization analysis of pancreatic adenocarcinoma: identification of genetic indicators that predict patient outcome. Cancer Sci.2007; 98:392-400.
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